ArticleThe Biochemical journal2016
Selenophosphate synthetase 1 is an essential protein with roles in regulation of redox homoeostasis in mammals.
Article in The Biochemical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Genetics of selenoproteins and selenoprotein metabolism - An overview of current concepts and emerging aspects.Redox biology · 2026Review
- De novo missense variants in exon 9 of SEPHS1 cause a neurodevelopmental condition with developmental delay, poor growth, hypotonia, and dysmorphic features.American journal of human genetics · 2024Article
- Systematic Analysis of Genes Related to Selenium Bioaccumulation in Microalgae: A Review.Biology · 2023Review
- Selenium in Bodily Homeostasis: Hypothalamus, Hormones, and Highways of Communication.International journal of molecular sciences · 2022Review
- SEPHS1: Its evolution, function and roles in development and diseases.Archives of biochemistry and biophysics · 2022Article
- SPS1 deficiency-triggered PGRP-LC and Toll expression controls innate immunity in Drosophila S2 cells.Biology open · 2022Article
- Selenocysteine Machinery Primarily Supports TXNRD1 and GPX4 Functions and Together They Are Functionally Linked with SCD and PRDX6.Biomolecules · 2022Article
- Article
- Regulation of A-to-I RNA editing and stop codon recoding to control selenoprotein expression during skeletal myogenesis.Nature communications · 2022Article
- Widespread occurrence of covalent lysine-cysteine redox switches in proteins.Nature chemical biology · 2022Article
- Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis by dysregulating redox homeostasis.Nature communications · 2022Article
- Identification of Signaling Pathways for Early Embryonic Lethality and Developmental Retardation inInternational journal of molecular sciences · 2021Article
- Constitutive Oxidative Stress by SEPHS1 Deficiency Induces Endothelial Cell Dysfunction.International journal of molecular sciences · 2021Article
- SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.Experimental hematology & oncology · 2021Article
- Trypanosomatid selenophosphate synthetase structure, function and interaction with selenocysteine lyase.PLoS neglected tropical diseases · 2020Article
- Tolerance to Selenoprotein Loss Differs between Human and Mouse.Molecular biology and evolution · 2020Article
- Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo.Nature communications · 2019Article
- Selenocysteine β-Lyase: Biochemistry, Regulation and Physiological Role of the Selenocysteine Decomposition Enzyme.Antioxidants (Basel, Switzerland) · 2019Review
- A Genetic Screen Using theG3 (Bethesda, Md.) · 2019Article
- Toenail selenium, genetic variation in selenoenzymes and risk and outcome in glioma.Cancer epidemiology · 2018Article
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Authors and funding
19 authors at 2 institutions in 2 countries.
Funding
Abstract
Selenophosphate synthetase (SPS) was initially detected in bacteria and was shown to synthesize selenophosphate, the active selenium donor. However, mammals have two SPS paralogues, which are designated SPS1 and SPS2. Although it is known that SPS2 catalyses the synthesis of selenophosphate, the function of SPS1 remains largely unclear. To examine the role of SPS1 in mammals, we generated a Sps1-knockout mouse and found that systemic SPS1 deficiency led to embryos that were clearly underdeveloped by embryonic day (E)8.5 and virtually resorbed by E14.5. The knockout of Sps1 in the liver preserved viability, but significantly affected the expression of a large number of mRNAs involved in cancer, embryonic development and the glutathione system. Particularly notable was the extreme deficiency of glutaredoxin 1 (GLRX1) and glutathione transferase Omega 1 (GSTO1). To assess these phenotypes at the cellular level, we targeted the removal of SPS1 in F9 cells, a mouse embryonal carcinoma (EC) cell line, which affected the glutathione system proteins and accordingly led to the accumulation of hydrogen peroxide in the cell. Furthermore, we found that several malignant characteristics of SPS1-deficient F9 cells were reversed, suggesting that SPS1 played a role in supporting and/or sustaining cancer. In addition, the overexpression of mouse or human GLRX1 led to a reversal of observed increases in reactive oxygen species (ROS) in the F9 SPS1/GLRX1-deficient cells and resulted in levels that were similar to those in F9 SPS1-sufficient cells. The results suggested that SPS1 is an essential mammalian enzyme with roles in regulating redox homoeostasis and controlling cell growth.
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