ArticleMalaria journal2016
PCR detection of malaria parasites and related haemosporidians: the sensitive methodology in determining bird-biting insects.
Article in Malaria journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Haemosporidian parasites in Kansas birds: molecular and histological evidence implicating Culicoides haematopotus (Diptera: Ceratopogonidae) in transmission.Journal of medical entomology · 2026Article
- A literature review on the role of Culicoides in the transmission of avian blood parasites in Europe.Parasites & vectors · 2025Review
- Combining blood meal analysis and parasite detection yields a more comprehensive understanding of insect host feeding patterns.Parasites & vectors · 2025Article
- Novel Insight intoPathogens (Basel, Switzerland) · 2025Article
- An Overview of the Neglected Modes of Existence in Avian Haemosporidian Parasites.Microorganisms · 2025Review
- A Literature Review on the Role of the InvasiveAnimals : an open access journal from MDPI · 2024Review
- High Abundance ofInsects · 2024Article
- Insights into the Biology ofMicroorganisms · 2023Review
- Phylogeographic Patterns of Haemoproteid Assemblages of Selected Avian Hosts: Ecological and Evolutionary Implications.Microorganisms · 2022Article
- Article
- Vector incrimination and transmission of avian malaria at an aquarium in Japan: mismatch in parasite composition between mosquitoes and penguins.Malaria journal · 2021Article
- Culicoides biting midges involved in transmission of haemoproteids.Parasites & vectors · 2021Article
- Identification of a new vector species of avian haemoproteids, with a description of methodology for the determination of natural vectors of haemosporidian parasites.Parasites & vectors · 2019Article
- Characterization of Plasmodium relictum, a cosmopolitan agent of avian malaria.Malaria journal · 2018Article
- The widespread biting midge Culicoides impunctatus (Ceratopogonidae) is susceptible to infection with numerous Haemoproteus (Haemoproteidae) species.Parasites & vectors · 2017Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundKnowledge about feeding preference of blood-sucking insects is important for the better understanding epidemiology of vector-borne parasitic diseases. Extraction of DNA from blood present in abdomens of engorged insects provides opportunities to identify species of their vertebrate hosts. However, this approach often is insufficiently sensitive due to rapid degeneration of host DNA in midguts. Recent studies indicate that avian malaria parasites (Plasmodium spp.) and related haemosporidians (Haemosporida) belonging to Haemoproteus can persist both in vectors and resistant blood-sucking insects for several weeks after initial blood meals, and these parasites can be readily detected by polymerase chain reaction (PCR)-based methods. Because avian haemosporidians are cosmopolitan, prevalent and strictly specific to birds, the determination of haemosporidian DNA in blood-sucking dipterans can be used as molecular tags in determining bird-biting insects. This hypothesis was tested by investigation of prevalence of natural haemosporidian infections in wild-caught mosquitoes (Culicidae) and biting midges (Ceratopogonidae: Culicoides).
resultsFemales of mosquitoes (1072 individuals of three species) and biting midges (300 individuals of three species) were collected in wildlife using simple netting. They were identified and tested individually for the presence of both the haemosporidian parasites and the bird blood using PCR-based methods. Seven different Haemoproteus and two Plasmodium lineages were detected, with overall infection prevalence of 1.12 and 1.67 % in mosquitoes and biting midges, respectively. In all, the detection rate of avian haemosporidian parasites was three fold higher compared with the detection of avian blood.
conclusionsMolecular markers of avian malaria parasites and other haemosporidians are recommended for getting additional knowledge about blood-sucking dipterans feeding on bird blood. Many genetic lineages of avian haemosporidians are specific to avian hosts, therefore, the detection of these parasite lineages in blood-sucking insects can indicate their feeding preferences on the level of species or groups of related bird species.
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