Evidence map›Paper›PMID 27206851›Full record

ArticleTranslational stroke research2016

Microglia Activation and Polarization After Intracerebral Hemorrhage in Mice: the Role of Protease-Activated Receptor-1.

Shu Wan, Yingying Cheng, Hang Jin, Dewei Guo, Ya Hua, Richard F Keep, Guohua Xi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational stroke research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
96citing papers in PubMed, 1 pooled it
13.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

96 citing papers in PubMed, 1 synthesis or guideline pooled it, 141 citations in OpenAlex.

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  17. The Roles of RhoA/ROCK/NF-κB Pathway in Microglia Polarization Following Ischemic Stroke.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2024
    Review
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36 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Shu WanDepartment of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA.
Yingying ChengDepartment of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA.
Hang JinDepartment of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA.
Dewei GuoDepartment of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA.
Ya HuaDepartment of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA.
Richard F KeepDepartment of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA.
Guohua XiDepartment of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA. guohuaxi@umich.edu.
University of Michigan–Ann Arbor · USJilin University · CN

Funding

Iron, minocycline and brain injury after intracerebral hemorrhageR01NS090925 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI XI, GUOHUA · 2015 to 2019
$1.7M
Targeting CD47 to aid in clearing intracerebral hemorrhagesR01NS096917 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HUA, YA, XI, GUOHUA · 2016 to 2020
$1.7M
Mechanisms of Brain Injury after Intraventricular HemorrhageR01NS073595 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI XI, GUOHUA · 2012 to 2016
$1.7M
Mechanisms of early brain injury after subarachnoid hemmorrhageR01NS079157 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI XI, GUOHUA · 2012 to 2016
$1.7M
Lipocalin-2 and Intracerebral Hemorrhage-induced Brain InjuryR21NS091545 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HUA, YA · 2015 to 2016
$427k
CD47 and hematoma clearance in intracerebral hemorrhageR21NS084049 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HUA, YA · 2013 to 2014
$425k
NINDS NIH HHS R01 NS073595NINDS NIH HHS R01 NS079157NINDS NIH HHS R01 NS090925NINDS NIH HHS R01 NS096917NINDS NIH HHS R21 NS084049NINDS NIH HHS R21 NS091545
6 · The paper itself

Abstract

Polarized microglia play a dual (beneficial/detrimental) role in neurological diseases. However, the status and the factors that modulate microglia polarization in intracerebral hemorrhage (ICH) remain unclear. In the present study, we investigated the role of protease-activated receptor-1 (PAR-1, a thrombin receptor) in ICH-induced microglia polarization in mice. Male wild-type (WT) and PAR-1 knockout (PAR-1 KO) mice received an infusion of 30-μL autologous blood or saline into the right basal ganglia. Mice were euthanized at different time points and the brains were used for Western blotting and immunohistochemistry. Some mice had magnetic resonance imaging. We found that ICH induced microglia activation and polarization. M1 phenotypic markers were markedly increased and reached a peak as early as 4 h, remained high at 3 days and decreased 7 days after ICH. M2 phenotypic markers were upregulated later than M1 markers reaching a peak at day 1 and declining by day 7 after ICH. PAR-1 was upregulated after ICH and expressed in the neurons and microglia. ICH induced less brain swelling and neuronal death in PAR-1 KO mice, and this was associated with less M1 polarization and reduced proinflammatory cytokine levels in the brain. In conclusion, these results suggest that polarized microglia occur dynamically after ICH and that PAR-1 plays a role in the microglia activation and polarization.

Indexed as

AnimalsBasal GangliaCell CountCell PolarityCerebral HemorrhageCytokinesDisease Models, AnimalDoxorubicinFluoresceinsGene Expression RegulationGlial Fibrillary Acidic ProteinInterleukin-10Lectins, C-TypeMacrophagesMagnetic Resonance ImagingMaleCytokinesDoxorubicinFluoresceinsfluoro-jade CGlial Fibrillary Acidic ProteinInterleukin-10Lectins, C-TypeMannose-Binding LectinsMannose ReceptorMatrix Metalloproteinase 9N-(3-pyridylmethyl)adriamycinNitric Oxide Synthase Type IIOligopeptidesPAR-1-activating peptideReceptor, PAR-1Receptors, Cell SurfaceReceptors, IgGTransforming Growth Factor betaCerebral hemorrhageMicrogliaMouseProtease-activated receptor-1

Identifiers

PMID27206851
PMCPMC5065741
OpenAlexW2405860079

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.