ArticleGenetics2016
Phenotypic Consequences of a Spontaneous Loss of Heterozygosity in a Common Laboratory Strain of Candida albicans.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Probing gene function inmSphere · 2024Article
- CRISPR-Cas9 Editing Induces Loss of Heterozygosity in the Pathogenic Yeast Candida parapsilosis.mSphere · 2022Article
- Multiple Stochastic Parameters Influence Genome Dynamics in a Heterozygous Diploid Eukaryotic Model.Journal of fungi (Basel, Switzerland) · 2022Article
- Review
- Genetic and Phenotypic Diversities in Experimental Populations of Diploid Inter-Lineage Hybrids in the Human PathogenicMicroorganisms · 2021Article
- Factors that influence bidirectional long-tract homozygosis due to double-strand break repair in Candida albicans.Genetics · 2021Article
- The Genetic Architecture of Emerging Fungicide Resistance in Populations of a Global Wheat Pathogen.Genome biology and evolution · 2020Article
- Use of CRISPR-Cas9 To Target Homologous Recombination Limits Transformation-Induced Genomic Changes in Candida albicans.mSphere · 2020Article
- The Impact of Gene Dosage and Heterozygosity on The Diploid PathobiontJournal of fungi (Basel, Switzerland) · 2019Review
- Mechanisms of genome evolution in Candida albicans.Current opinion in microbiology · 2019Review
- Partner Choice in Spontaneous Mitotic Recombination in Wild Type and Homologous Recombination Mutants ofG3 (Bethesda, Md.) · 2019Article
- Adaptation by Loss of Heterozygosity inGenetics · 2019Article
- Susceptibility to Medium-Chain Fatty Acids Is Associated with Trisomy of Chromosome 7 inmSphere · 2019Article
- Article
- Diverse Lineages ofGenetics · 2019Article
- Article
- Comparative molecular dynamics studies of heterozygous open reading frames of DNA polymerase eta (η) in pathogenic yeast Candida albicans.Scientific reports · 2017Article
- Ploidy dynamics and evolvability in fungi.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By testing the susceptibility to DNA damaging agents of several Candida albicans mutant strains derived from the commonly used laboratory strain, CAI4, we uncovered sensitivity to methyl methanesulfonate (MMS) in CAI4 and its derivatives, but not in CAF2-1. This sensitivity is not a result of URA3 disruption because the phenotype was not restored after URA3 reintroduction. Rather, we found that homozygosis of a short region of chromosome 3R (Chr3R), which is naturally heterozygous in the MMS-resistant-related strains CAF4-2 and CAF2-1, confers MMS sensitivity and modulates growth polarization in response to MMS. Furthermore, induction of homozygosity in this region in CAF2-1 or CAF4-2 resulted in MMS sensitivity. We identified 11 genes by SNP/comparative genomic hybridization containing only the a alleles in all the MMS-sensitive strains. Four candidate genes, SNF5, POL1, orf19.5854.1, and MBP1, were analyzed by generating hemizygous configurations in CAF2-1 and CAF4-2 for each allele of all four genes. Only hemizygous MBP1a/mbp1b::SAT1-FLIP strains became MMS sensitive, indicating that MBP1a in the homo- or hemizygosis state was sufficient to account for the MMS-sensitive phenotype. In yeast, Mbp1 regulates G1/S genes involved in DNA repair. A second region of homozygosis on Chr2L increased MMS sensitivity in CAI4 (Chr3R homozygous) but not CAF4-2 (Chr3R heterozygous). This is the first example of sign epistasis in C. albicans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.