ArticleTissue engineering. Part C, Methods2016
In Vitro Endothelialization of Biodegradable Vascular Grafts Via Endothelial Progenitor Cell Seeding and Maturation in a Tubular Perfusion System Bioreactor.
Article in Tissue engineering. Part C, Methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Assessment of the healing process after percutaneous implantation of a cardiovascular device: a systematic review.The international journal of cardiovascular imaging · 2020Pooled it
- Perfusion Bioreactor Conditioning of Small-diameter Plant-based Vascular Grafts.Tissue engineering and regenerative medicine · 2024Article
- Integrating Computational and Biological Hemodynamic Approaches to Improve Modeling of Atherosclerotic Arteries.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Biomimetic Approaches in Scaffold-Based Blood Vessel Tissue Engineering.Biomimetics (Basel, Switzerland) · 2024Review
- Use of mesenchymal stem cells to enhance or restore fertility potential: a systematic review of available experimental strategies.Human reproduction open · 2023Review
- Personalized tissue-engineered arteries as vascular graft transplants: A safety study in sheep.Regenerative therapy · 2022Article
- Development of Liver-on-Chip Integrating a Hydroscaffold Mimicking the Liver's Extracellular Matrix.Bioengineering (Basel, Switzerland) · 2022Article
- Pulmonary artery augmentation and aortic valve repair using novel tissue-engineered grafts.JTCVS techniques · 2022Article
- Vascular implants - new aspects for in situ tissue engineering.Engineering in life sciences · 2022Review
- Tissue engineering: Relevance to neonatal congenital heart disease.Seminars in fetal & neonatal medicine · 2022Review
- Facilitation of adhesion and spreading of endothelial cells on silicone oxide-coated dacron material by microwave-excited low-pressure plasma.Innovative surgical sciences · 2022Article
- Evaluation of the Feasibility of Endothelial Colony-Forming Cells to Develop Tissue-Engineered Vascular Grafts Based on the Gene Expression Profile Analysis.Sovremennye tekhnologii v meditsine · 2022Article
- The path to a hemocompatible cardiovascular implant: Advances and challenges of current endothelialization strategies.Frontiers in cardiovascular medicine · 2022Review
- Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Strategies to counteract adverse remodeling of vascular graft: A 3D view of current graft innovations.Frontiers in bioengineering and biotechnology · 2022Review
- Surface modification of decellularized bovine carotid arteries with human vascular cells significantly reduces their thrombogenicity.Journal of biological engineering · 2021Article
- Patient-Specific 3D Bioprinted Models of Developing Human Heart.Advanced healthcare materials · 2021Article
- Comparative Evaluation on Impacts of Fibronectin, Heparin-Chitosan, and Albumin Coating of Bacterial Nanocellulose Small-Diameter Vascular Grafts on Endothelialization In Vitro.Nanomaterials (Basel, Switzerland) · 2021Article
- In vitro recellularization of decellularized bovine carotid arteries using human endothelial colony forming cells.Journal of biological engineering · 2021Article
- Tissue Engineered Vascular Graft Recipient Interleukin 10 Status Is Critical for Preventing Thrombosis.Advanced healthcare materials · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A critical challenge to the success of biodegradable vascular grafts is the establishment of a healthy endothelium. To establish this monolayer of endothelial cells (ECs), a variety of techniques have been developed, including cell seeding. Vascular grafts may be seeded with relevant cell types and allowed to mature before implantation. Due to the low proliferative ability of adult ECs and issues with donor site morbidity, there has been increasing interest in using endothelial progenitor cells (EPCs) for vascular healing procedures. In this work, we combined the proliferative and differentiation capabilities of a commercial cell line of early EPCs with an established bioreactor system to support the maturation of cell-seeded vascular grafts. All components of the vascular graft and bioreactor setup are commercially available and allow for complete customization of the scaffold and culturing system. This bioreactor setup enables the control of flow through the graft, imparting fluid shear stress on EPCs and affecting cellular proliferation and differentiation. Grafts cultured with EPCs in the bioreactor system demonstrated greatly increased cell populations and neotissue formation compared with grafts seeded and cultured in a static system. Increased expression of markers for mature endothelial tissues were also observed in bioreactor-cultured EPC-seeded grafts. These findings suggest the distinct advantages of a customizable bioreactor setup for the proliferation and maturation of EPCs. Such a strategy may be beneficial for utilizing EPCs in vascular tissue engineering applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.