ArticleParasites & vectors2016
Rickettsia parkeri colonization in Amblyomma maculatum: the role of superoxide dismutases.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- mGem: A tale as old as blood-do tick-borne pathogens exploit arthropod antioxidant defenses?mBio · 2026Review
- An Nrf2-Nup214 positive feedback loop sustains the antioxidant response and promotes microbial infection in ticks.bioRxiv : the preprint server for biology · 2026Article
- Insecticide resistant Anopheles from Ethiopia but not Burkina Faso show a microbiota composition shift upon insecticide exposure.Parasites & vectors · 2025Article
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- PERK-mediated antioxidant response is key for pathogen persistence in ticks.bioRxiv : the preprint server for biology · 2023Article
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- Ambivalent Roles of Oxidative Stress in Triangular Relationships among Arthropod Vectors, Pathogens and Hosts.Antioxidants (Basel, Switzerland) · 2022Review
- Protein profiling of hemolymph in Haemaphysalis flava ticks.Parasites & vectors · 2022Article
- Redox Imbalance and Its Metabolic Consequences in Tick-Borne Diseases.Frontiers in cellular and infection microbiology · 2022Review
- Hematophagy and tick-borne Rickettsial pathogen shape the microbial community structure and predicted functions within the tick vector,Frontiers in cellular and infection microbiology · 2022Article
- Recent advances in understanding tick and rickettsiae interactions.Parasite immunology · 2021Review
- Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches.BMC genomics · 2020Article
- Is selenoprotein K required for Borrelia burgdorferi infection within the tick vector Ixodes scapularis?Parasites & vectors · 2019Article
- Vector Tick Transmission Model of Spotted Fever Rickettsiosis.The American journal of pathology · 2019Article
- Article
- Amblyomma maculatum SECIS binding protein 2 and putative selenoprotein P are indispensable for pathogen replication and tick fecundity.Insect biochemistry and molecular biology · 2017Article
- A proteomic insight into the midgut proteome of Ornithodoros moubata females reveals novel information on blood digestion in argasid ticks.Parasites & vectors · 2017Article
- Catalase is a determinant of the colonization and transovarial transmission of Rickettsia parkeri in the Gulf Coast tick Amblyomma maculatum.Insect molecular biology · 2017Article
- Microbial Communities in North American Ixodid Ticks of Veterinary and Medical Importance.Frontiers in veterinary science · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe Gulf Coast tick (Amblyomma maculatum) is an arthropod vector of Rickettsia parkeri, the causative agent of American boutonneuse fever and an infectious agent of public health significance. In this study, we evaluated the biological significance of the superoxide dismutases (SODs) of A. maculatum in hematophagy and R. parkeri colonization within the tick host.
methodsAn RNA interference approach was used to measure the functional roles of tick SODs (Cu/Zn-SOD and Mn-SOD) in R. parkeri colonization of the tick vector. Total microbial load, R. parkeri infection rate, and compensatory mechanisms by tick genes were examined using quantitative polymerase chain reaction (PCR) and quantitative reverse-transcriptase PCR assays. SOD enzymatic activity assays and malondialdehyde (MDA) lipid peroxidation were employed to determine the redox states in the tick tissues.
resultsKnockdown of the Cu/Zn-SOD gene caused the upregulation of Mn-SOD in transcript levels. Single and dual knockdowns of the SOD genes caused an increase in MDA lipid peroxidation while SOD enzymatic activities did not show a significant change. Mn-SOD knockdown resulted in a substantial increase in the microbial load; however, Cu/Zn-SOD transcript depletion prompted an upsurge in the midgut bacterial load, and significantly decreased the bacterial load in salivary gland tissues. Additionally, Cu/Zn-SOD transcript silencing led to significantly fewer R. parkeri DNA copy numbers in both tick tissues (midguts and salivary glands).
conclusionsSOD enzymes play an important function in the regulation of bacterial communities associated with tick vectors and also in the defense mechanisms against the damage caused by reactive oxygen species within the tick. Knockdown experiments increased the levels of total oxidative stress in ticks, revealing the interplay between SOD isozymes that results in the transcriptional regulation of tick antioxidants. Moreover, the tick's Cu/Zn-SOD aids in the colonization of R. parkeri in tick tissues providing evidence of A. maculatum's vectorial success for a spotted fever group rickettsial pathogen.
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Registered trials
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