ArticleInhalation toxicology2016
Differential expression of pro-inflammatory and oxidative stress mediators induced by nitrogen dioxide and ozone in primary human bronchial epithelial cells.
Article in Inhalation toxicology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Toxicological Effects of Air Pollutants on Human Airway Cell Models Using Air-liquid Interface Systems: A Systematic Review.Current environmental health reports · 2025Pooled it
- Effects of prenatal exposure to NOEnvironmental science and pollution research international · 2020Pooled it
- Cardiorespiratory health effects of gaseous ambient air pollution exposure in low and middle income countries: a systematic review and meta-analysis.Environmental health : a global access science source · 2018Pooled it
- Cross-sectional evaluation of exposure to ozone, nitrogen dioxide, and particulate mass levels on circulating immune markers in women in the California Teachers Study.Scientific reports · 2025Article
- Assessing Carbon Monoxide Exposure in Food Delivery Workers by Using Exhaled Air with Consideration of Smoking Habits.Journal of epidemiology and global health · 2025Article
- Redox Regulation of Microvascular Physiology and Pathophysiology: Insights into Therapeutic Strategies and Limitations.Antioxidants & redox signaling · 2025Review
- Cross-sectional evaluation of exposure to ozone, nitrogen dioxide, and particulate mass levels on circulating immune markers in women in the California Teachers Study.Research square · 2025Article
- The Impact of NOJournal of applied toxicology : JAT · 2025Article
- Impact of multiple environmental factors on influenza-like illness in Fujian Province, China, 2015-2023: a multicity study.Frontiers in public health · 2025Article
- The New Paradigm: The Role of Proteins and Triggers in the Evolution of Allergic Asthma.International journal of molecular sciences · 2024Review
- Article
- Severe acute respiratory syndrome coronaviruses contributing to mitochondrial dysfunction: Implications for post-COVID complications.Mitochondrion · 2023Review
- Short-term association of CO and NOFrontiers in public health · 2023Article
- Beyond allergic progression: From molecules to microbes as barrier modulators in the gut-lung axis functionality.Frontiers in allergy · 2023Review
- Long-term air pollution exposure and incident stroke in American older adults: A national cohort study.Global epidemiology · 2022Article
- Acute exposure to air pollutants increase the risk of acute glaucoma.BMC public health · 2022Article
- Association between air pollution exposure and coronary heart disease hospitalization in a humid sub-tropical region of China: A time-series study.Frontiers in public health · 2022Article
- The Association between Exposure to Air Pollution and Type 1 Diabetes Mellitus: A Systematic Review and Meta-Analysis.Advanced biomedical research · 2022Article
- Higher ambient nitrogen dioxide is associated with an elevated risk of hospital-acquired acute kidney injury.Clinical kidney journal · 2022Article
- Association of coronavirus pathogencity with the level of antioxidants and immune system.Journal of family medicine and primary care · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
contextNO2 and O3 are ubiquitous air toxicants capable of inducing lung damage to the respiratory epithelium. Due to their oxidizing capabilities, these pollutants have been proposed to target specific biological pathways, but few publications have compared the pathways activated.
objectiveThis work will test the premise that NO2 and O3 induce toxicity by activating similar cellular pathways.
methodsPrimary human bronchial epithelial cells (HBECs, n = 3 donors) were exposed for 2 h at an air-liquid interface to 3 ppm NO2, 0.75 ppm O3, or filtered air and harvested 1 h post-exposure. To give an overview of pathways that may be influenced by each exposure, gene expression was measured using PCR arrays for toxicity and oxidative stress. Based on the results, genes were selected to quantify whether expression changes were changed in a dose- and time-response manner using NO2 (1, 2, 3, or 5 ppm), O3 (0.25, 0.50, 0.75, or 1.00 ppm), or filtered air and harvesting 0, 1, 4 and 24 h post-exposure.
resultsUsing the arrays, genes related to oxidative stress were highly induced with NO2 while expression of pro-inflammatory and vascular function genes was found subsequent to O3. NO2 elicited the greatest HMOX1 response, whereas O3 more greatly induced IL-6, IL-8 and PTGS2 expression. Additionally, O3 elicited a greater response 1 h post-exposure and NO2 produced a maximal response after 4 h.
conclusionWe have demonstrated that these two oxidant gases stimulate differing mechanistic responses in vitro and these responses occur at dissimilar times.
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Registered trials
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