ArticleBiomaterials2016
Effects of substrate stiffness and cell-cell contact on mesenchymal stem cell differentiation.
Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
106 citing papers in PubMed, 1 synthesis or guideline pooled it, 267 citations in OpenAlex.
- Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020Pooled it
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- Ribbon-shaped microgels as bioinks for 3D bioprinting of anisotropic tissue structures.Bioactive materials · 2026Article
- Mechanobiology in Stem Cell-Based Bioprinting.Cell proliferation · 2026Review
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- The Bionic Interface: Considering the Material Mediated Electrical Stimulation of Stem Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Adipose stromal cells in the human rotator cuff are resistant to fibrotic microenvironmental cues.The Journal of physiology · 2025Article
- At the Pointy End of Mechanobiology: AFM for Transient Biomechanical Analysis.Advanced healthcare materials · 2025Review
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- Covalent Peptide-Graphene Conjugates for Enhanced Cell Spreading, Osteogenic Differentiation, and Angiogenesis in Bone Defects.Chembiochem : a European journal of chemical biology · 2025Article
- Synthesis and use of thermoplastic polymers for tissue engineering purposes.International journal of pharmaceutics: X · 2025Review
- Osteogenic Potential of 3D-Printed Porous Poly(lactide-ACS applied materials & interfaces · 2025Article
- Self-propagating wave drives morphogenesis of skull bones in vivo.Nature communications · 2025Article
- Combinatorial strategy for engineering cartilage and bone microtissues using microfluidic cell-laden microgels.Biofabrication · 2025Article
- Combinatorial extracellular matrix tissue chips for optimizing mesenchymal stromal cell microenvironment and manufacturing.NPJ Regenerative medicine · 2025Article
- Microbead Encapsulation Strategy for Efficient Production of Extracellular Vesicles Derived From Human Mesenchymal Stem Cells.Journal of extracellular vesicles · 2025Article
- Ultrasound-generated bubbles enhance osteogenic differentiation of mesenchymal stromal cells in composite collagen hydrogels.Bioactive materials · 2025Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The mechanical properties of the microenvironment and direct contact-mediated cell-cell interactions are two variables known to be important in the determination of stem cell differentiation fate, but little is known about the interplay of these cues. Here, we use a micropatterning approach on polyacrylamide gels of tunable stiffnesses to study how homotypic cell-cell contacts and mechanical stiffness affect different stages of osteogenesis of mesenchymal stem cells (MSCs). Nuclear localization of transcription factors associated with osteogenesis depended on substrate stiffness and was independent of the degree of cell-cell contact. However, expression of alkaline phosphatase, an early protein marker for osteogenesis, increased only in cells with both direct contact with neighboring cells and adhesion to stiffer substrates. Finally, mature osteogenesis, as assessed by calcium deposition, was low in micropatterned cells, even on stiff substrates and in multicellular clusters. These results indicate that substrate stiffness and the presence of neighboring cells regulate osteogenesis in MSCs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.