Evidence map›Paper›PMID 27199968›Full record

ReviewFrontiers in microbiology2016

Advances in Molecular Serotyping and Subtyping of Escherichia coli.

Pina M Fratamico, Chitrita DebRoy, Yanhong Liu, David S Needleman, Gian Marco Baranzoni, Peter Feng

Abstract readReview
In one paragraph

Review in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
96citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

96 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Complete genomes ofMicrobiology resource announcements · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Antimicrobial resistantOne health (Amsterdam, Netherlands) · 2026
    Review
  8. Article
  9. Article
  10. Review
  11. High Prevalence of Multidrug-Resistant HaemolyticAntibiotics (Basel, Switzerland) · 2026
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Investigating the novel Escherichia coli bacteriophage Jgk1 as a potential antimicrobial agent.International microbiology : the official journal of the Spanish Society for Microbiology · 2025
    Article
  17. Review
  18. Genomic Markers Distinguishing Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2025
    Article
  19. Article
  20. Article

36 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Pina M FratamicoEastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor PA, USA.
Chitrita DebRoyDepartment of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park PA, USA.
Yanhong LiuEastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor PA, USA.
David S NeedlemanEastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor PA, USA.
Gian Marco BaranzoniEastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor PA, USA.
Peter FengDivision of Microbiology, U.S. Food and Drug Administration, College Park MD, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Escherichia coli plays an important role as a member of the gut microbiota; however, pathogenic strains also exist, including various diarrheagenic E. coli pathotypes and extraintestinal pathogenic E. coli that cause illness outside of the GI-tract. E. coli have traditionally been serotyped using antisera against the ca. 186 O-antigens and 53 H-flagellar antigens. Phenotypic methods, including bacteriophage typing and O- and H- serotyping for differentiating and characterizing E. coli have been used for many years; however, these methods are generally time consuming and not always accurate. Advances in next generation sequencing technologies have made it possible to develop genetic-based subtyping and molecular serotyping methods for E. coli, which are more discriminatory compared to phenotypic typing methods. Furthermore, whole genome sequencing (WGS) of E. coli is replacing established subtyping methods such as pulsed-field gel electrophoresis, providing a major advancement in the ability to investigate food-borne disease outbreaks and for trace-back to sources. A variety of sequence analysis tools and bioinformatic pipelines are being developed to analyze the vast amount of data generated by WGS and to obtain specific information such as O- and H-group determination and the presence of virulence genes and other genetic markers.

Indexed as

detectionEscherichia coliH-typeidentificationmolecular serotypingO-groupsubtypingwhole genome sequencing

Identifiers

PMID27199968
PMCPMC4853403

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.