ArticleFrontiers in microbiology2016
Prevalence of Escherichia coli Virulence Genes in Patients with Diarrhea and a Subpopulation of Healthy Volunteers in Madrid, Spain.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Molecular identification of the most frequent pathotypes ofOpen veterinary journal · 2024Article
- Global Dynamics of Gastrointestinal Colonisations and Antimicrobial Resistance: Insights from International Travellers to Low- and Middle-Income Countries.Tropical medicine and infectious disease · 2024Article
- Short reads-based characterization of pathotype diversity and drug resistance among Escherichia coli isolated from patients attending regional referral hospitals in Tanzania.BMC medical genomics · 2024Article
- Isolation and characterization of lytic bacteriophages from various sources in Addis Ababa against antimicrobial-resistant diarrheagenic Escherichia coli strains and evaluation of their therapeutic potential.BMC infectious diseases · 2024Article
- Biofilm-forming antimicrobial-resistant pathogenicHeliyon · 2023Article
- Diarrhoeal pathogens in the stools of children living with HIV in Ibadan, Nigeria.Frontiers in cellular and infection microbiology · 2023Article
- Virulence Factors and Antimicrobial Resistance of UropathogenicBioMed research international · 2023Article
- Isolation and Characterization of Lytic Bacteriophages Active against Clinical Strains ofViruses · 2022Article
- Microbiology and Epidemiology ofMicroorganisms · 2022Review
- A Method to Determine the Efficacy of a Commercial Phage Preparation against Uropathogens in Urine and Artificial Urine Determined by Isothermal Microcalorimetry.Microorganisms · 2022Article
- Occurrence of Hybrid DiarrhoeagenicMicroorganisms · 2021Article
- Frequency of k99, stx1, and stx2 Virulence Factors in Escherichia coli isolated from Diarrheic and Clinically Healthy Suckling Calves in Sistan and Baluchistan Province, Iran.Archives of Razi Institute · 2021Article
- Profiling Virulence and Antimicrobial Resistance Markers of EnterovirulentOsong public health and research perspectives · 2020Article
- Characterization and Lytic Activity of IsolatedIranian journal of medical sciences · 2020Article
- Mixed Aetiology of Diarrhoea in Infants Attending Clinics in the North-West Province of South Africa: Potential for Sub-Optimal Treatment.Pathogens (Basel, Switzerland) · 2020Article
- Novel genotypes and multilocus genotypes of Enterocytozoon bieneusi in two wild rat species in China: potential for zoonotic transmission.Parasitology research · 2020Article
- Still Something to Discover: Novel Insights intoViruses · 2019Article
- DiarrheagenicFrontiers in public health · 2019Article
- Sequential Combined Effect of Phages and Antibiotics on the Inactivation ofMicroorganisms · 2018Article
- Increased prevalence of pathogenic bacteria in the gut microbiota of infants at risk of developing celiac disease: The PROFICEL study.Gut microbes · 2018Article
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6 authors.
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Abstract
Etiological diagnosis of diarrheal diseases may be complicated by their multi-factorial nature. In addition, Escherichia coli strains present in the gut can occasionally harbor virulence genes (VGs) without causing disease, which complicates the assessment of their clinical significance in particular. The aim of this study was to detect and quantify nine VGs (stx1, stx2, eae, aggR, ehxA, invA, est, elt and bfpA) typically present in five E. coli enteric pathotypes [enterohaemorrhagic E. coli (EHEC), enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), and enteroinvasive E. coli (EIEC)] in fecal samples collected from 49 patients with acute diarrhea and 32 healthy controls from Madrid, Spain. In addition, the presence of four serotype-related genes (wzx O104 and fliCH4, rfb O157, and fliCH7) was also determined. Presence of target genes was assessed using a quantitative real-time PCR assay previously developed, and the association of presence and burden of VGs with clinical disease and/or other risk factors was explored. Prevalence of ehxA [typically associated with Shigatoxin producing E. coli (STEC) and (EPEC), invA (EIEC), and the rfb O157+fliCH7 (STEC)] combination were significantly (p < 0.02) higher in the diarrheic group, while the wzx O104+fliCH4 combination was significantly (p = 0.014) more prevalent in the control group. On the other hand, eae was detected in more than 90% of the individuals in both patient and control populations, and it was not associated with bfpA, suggesting the absence of typical EPEC. No significant differences in the quantitative values were detected for any VG among study groups, but the difference in the load of aggR (EAEC) and invA in the patients with respect to the controls was close to the significance, suggesting a potential role of these VGs in the clinical signs observed when they are present at high levels.
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