ArticleFrontiers in microbiology2016
Virulence Characterisation of Salmonella enterica Isolates of Differing Antimicrobial Resistance Recovered from UK Livestock and Imported Meat Samples.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 52 citations in OpenAlex.
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- Protective Effect of Procyanidin-Rich Grape Seed Extract against Gram-Negative Virulence Factors.Antibiotics (Basel, Switzerland) · 2023Article
- A systematic review of experimental studies on Salmonella persistence in insects.NPJ science of food · 2023Review
- FromACS omega · 2023Review
- Activity of a Bacteriophage Cocktail to ControlPHAGE (New Rochelle, N.Y.) · 2023Article
- Pathogen genomics and phage-based solutions for accurately identifying and controllingFrontiers in microbiology · 2023Article
- Development of a Phage Cocktail to TargetPharmaceuticals (Basel, Switzerland) · 2022Article
- Presence of Tetracycline and Sulfonamide Resistance Genes inAntibiotics (Basel, Switzerland) · 2021Review
- A genomic epidemiological study shows that prevalence of antimicrobial resistance inMicrobial genomics · 2021Article
- Whole-Genome Sequencing ofFrontiers in microbiology · 2021Article
- Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa.Journal of the South African Veterinary Association · 2020Article
- Prevalence, Antimicrobial Resistance, Virulence Genes and Genetic Diversity ofMicroorganisms · 2020Article
- Characterizing Antimicrobial ResistantFrontiers in microbiology · 2020Article
- Detection and Molecular Identification ofPathogens (Basel, Switzerland) · 2019Article
- Article
- Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (Frontiers in microbiology · 2019Article
- Genome-scale metabolic reconstructions of multiple Salmonella strains reveal serovar-specific metabolic traits.Nature communications · 2018Article
- Galleria mellonella - a novel infection model for the Mycobacterium tuberculosis complex.Virulence · 2018Article
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Authors and funding
8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica is a foodborne zoonotic pathogen of significant public health concern. We have characterized the virulence and antimicrobial resistance gene content of 95 Salmonella isolates from 11 serovars by DNA microarray recovered from UK livestock or imported meat. Genes encoding resistance to sulphonamides (sul1, sul2), tetracycline [tet(A), tet(B)], streptomycin (strA, strB), aminoglycoside (aadA1, aadA2), beta-lactam (bla TEM), and trimethoprim (dfrA17) were common. Virulence gene content differed between serovars; S. Typhimurium formed two subclades based on virulence plasmid presence. Thirteen isolates were selected by their virulence profile for pathotyping using the Galleria mellonella pathogenesis model. Infection with a chicken invasive S. Enteritidis or S. Gallinarum isolate, a multidrug resistant S. Kentucky, or a S. Typhimurium DT104 isolate resulted in high mortality of the larvae; notably presence of the virulence plasmid in S. Typhimurium was not associated with increased larvae mortality. Histopathological examination showed that infection caused severe damage to the Galleria gut structure. Enumeration of intracellular bacteria in the larvae 24 h post-infection showed increases of up to 7 log above the initial inoculum and transmission electron microscopy (TEM) showed bacterial replication in the haemolymph. TEM also revealed the presence of vacuoles containing bacteria in the haemocytes, similar to Salmonella containing vacuoles observed in mammalian macrophages; although there was no evidence from our work of bacterial replication within vacuoles. This work shows that microarrays can be used for rapid virulence genotyping of S. enterica and that the Galleria animal model replicates some aspects of Salmonella infection in mammals. These procedures can be used to help inform on the pathogenicity of isolates that may be antibiotic resistant and have scope to aid the assessment of their potential public and animal health risk.
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