ArticleFrontiers in microbiology2016
Nitrogen Stimulates the Growth of Subsurface Basalt-associated Microorganisms at the Western Flank of the Mid-Atlantic Ridge.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 21 citations in OpenAlex.
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- Diversity of Mixotrophic Neutrophilic Thiosulfate- and Iron-Oxidizing Bacteria from Deep-Sea Hydrothermal Vents.Microorganisms · 2022Article
- Potential autotrophic carbon-fixer and Fe(II)-oxidizerFrontiers in microbiology · 2022Article
- Molting Alters the Microbiome, Immune Response, and Digestive Enzyme Activity in Mud Crab (mSystems · 2021Article
- Microorganisms from deep-sea hydrothermal vents.Marine life science & technology · 2021Review
- Deep microbial proliferation at the basalt interface in 33.5-104 million-year-old oceanic crust.Communications biology · 2020Article
- Nitrifier abundance and diversity peak at deep redox transition zones.Scientific reports · 2019Article
- Deep Microbial Colonization in Saponite-Bearing Fractures in Aged Basaltic Crust: Implications for Subsurface Life on Mars.Frontiers in microbiology · 2019Article
- Iron-oxidizing bacteria in marine environments: recent progresses and future directions.World journal of microbiology & biotechnology · 2018Review
- Hemolymph Microbiomes of Three Aquatic Invertebrates as Revealed by a New Cell Extraction Method.Applied and environmental microbiology · 2018Article
- Metagenomics Reveals Microbial Diversity and Metabolic Potentials of Seawater and Surface Sediment From a Hadal Biosphere at the Yap Trench.Frontiers in microbiology · 2018Article
- An efficient strategy usingHeliyon · 2017Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oceanic crust constitutes the largest aquifer system on Earth, and microbial activity in this environment has been inferred from various geochemical analyses. However, empirical documentation of microbial activity from subsurface basalts is still lacking, particularly in the cool (<25°C) regions of the crust, where are assumed to harbor active iron-oxidizing microbial communities. To test this hypothesis, we report the enrichment and isolation of crust-associated microorganisms from North Pond, a site of relatively young and cold basaltic basement on the western flank of the Mid-Atlantic Ridge that was sampled during Expedition 336 of the Integrated Ocean Drilling Program. Enrichment experiments with different carbon (bicarbonate, acetate, methane) and nitrogen (nitrate and ammonium) sources revealed significant cell growth (one magnitude higher cell abundance), higher intracellular DNA content, and increased Fe(3+)/ΣFe ratios only when nitrogen substrates were added. Furthermore, a Marinobacter strain with neutrophilic iron-oxidizing capabilities was isolated from the basalt. This work reveals that basalt-associated microorganisms at North Pond had the potential for activity and that microbial growth could be stimulated by in vitro nitrogen addition. Furthermore, iron oxidation is supported as an important process for microbial communities in subsurface basalts from young and cool ridge flank basement.
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