ArticleFrontiers in microbiology2016
Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 56 citations in OpenAlex.
- Enhancement Strategies for Bioelectrocatalytic Conversion of Organic Waste.Chem & bio engineering · 2026Review
- Genomic architecture and transcriptional regulation of cellulose degradation in the novel marine bacterium Pseudoxanthomonas sp. JC1303.Molecular genetics and genomics : MGG · 2026Article
- Neg1 overexpression in Trichoderma harzianum T4 enhanced its ability to produce spores and antagonistic activity against phytopathogenic fungi.Archives of microbiology · 2024Article
- Promoters and Synthetic Promoters in Trichoderma reesei.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Expression of cellobiose dehydrogenase gene inFrontiers in microbiology · 2024Article
- Heterologously Expressed Cellobiose Dehydrogenase Acts as Efficient Electron-Donor of Lytic Polysaccharide Monooxygenase for Cellulose Degradation inInternational journal of molecular sciences · 2023Article
- Strategies Shaping the Transcription of Carbohydrate-Active Enzyme Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- Synergistic hydrolysis of filter paper by recombinant cellulase cocktails leveraging a key cellobiase, Cba2, ofFrontiers in bioengineering and biotechnology · 2022Article
- Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei.Microbial cell factories · 2021Article
- Two new cellulolytic fungal species isolated from a 19Scientific reports · 2018Article
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Genetic engineering of Trichoderma reesei cellulases and their production.Microbial biotechnology · 2017Review
- Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems.Microbial cell factories · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent demands for the production of lignocellulose biofuels boosted research on cellulase. Hydrolysis efficiency and production cost of cellulase are two bottlenecks in "biomass to biofuels" process. The Trichoderma cellulase mixture is one of the most commonly used enzymes for cellulosic hydrolysis. During hydrolytic process cellobiose accumulation causes feedback inhibition against most cellobiohydrolases and endoglucanases. In this study, we demonstrated the synergism effects between cellobiose dehydrogenase (CDH) and cellulase both in vitro and in vivo. The CDH from Phanerochaete chrysosporium was heterologously expressed in Pichia pastoris. Supplementation of the purified CDH in Trichoderma cellulase increased the cellulase activities. Especially β-glucosidase activity was increased by 30-100% varying at different time points. On the other hand, the cdh gene was heterologously expressed in Trichoderma reesei to explore the synergism between CDH and cellulases in vivo. The analyses of gene expression and enzymatic profiles of filter paper activity, carboxymethylcellulase (CMCase) and β-glucosidase show the increased cellulase activity and the enhanced cellulase production in the cdh-expressing strains. The results elucidate a possible mechanism for diminishing the cellobiose inhibition of cellulase by CDH. These findings provide a novel perspective to make more economic enzyme cocktails for commercial application or explore alternative strategies for generating cellulase-producing strains with higher efficiency.
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