ArticleFrontiers in microbiology2016
Method for Indirect Quantification of CH4 Production via H2O Production Using Hydrogenotrophic Methanogens.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Quantitative analysis of amino acid excretion and consumption by Methanothermobacter marburgensis in fed-batch cultivation mode.Amino acids · 2026Article
- A high-pressure bioreactor system for the cultivation of Methanothermobacter marburgensis on advanced growth media for sustainable energy applications.Scientific reports · 2025Article
- Method for automated high performance closed batch cultivation of gas-utilizing methanogens.AMB Express · 2025Article
- Quantitative analysis of amino acid excretion by Methanothermobacter marburgensis under NScientific reports · 2025Article
- Comparison of Various Reducing Agents for Methane Production byMicroorganisms · 2023Article
- Analysis of biomass productivity and physiology ofFrontiers in microbiology · 2023Article
- Quantitative Analysis of Core Lipid Production inBioengineering (Basel, Switzerland) · 2022Article
- The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology.Microorganisms · 2022Review
- Scale-up of biomass production byFrontiers in microbiology · 2022Article
- SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system.Nature communications · 2021Article
- Hyperthermophilic methanogenic archaea act as high-pressure CHCommunications biology · 2021Article
- Membrane Lipid Composition and Amino Acid Excretion Patterns ofLife (Basel, Switzerland) · 2019Article
- Development of a simultaneous bioreactor system for characterization of gas production kinetics of methanogenic archaea at high pressure.Engineering in life sciences · 2019Article
- Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology.Folia microbiologica · 2019Review
- Physiology and methane productivity of Methanobacterium thermaggregans.Applied microbiology and biotechnology · 2018Article
- Biological methane production under putative Enceladus-like conditions.Nature communications · 2018Article
- The physiological effect of heavy metals and volatile fatty acids onBiotechnology for biofuels · 2018Article
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2 authors.
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Abstract
Hydrogenotrophic methanogens are an intriguing group of microorganisms from the domain Archaea. Methanogens exhibit extraordinary ecological, biochemical, and physiological characteristics and possess a huge biotechnological potential. Yet, the only possibility to assess the methane (CH4) production potential of hydrogenotrophic methanogens is to apply gas chromatographic quantification of CH4. In order to be able to effectively screen pure cultures of hydrogenotrophic methanogens regarding their CH4 production potential we developed a novel method for indirect quantification of the volumetric CH4 production rate by measuring the volumetric water production rate. This method was established in serum bottles for cultivation of methanogens in closed batch cultivation mode. Water production was estimated by determining the difference in mass increase in a quasi-isobaric setting. This novel CH4 quantification method is an accurate and precise analytical technique, which can be used to rapidly screen pure cultures of methanogens regarding their volumetric CH4 evolution rate. It is a cost effective alternative determining CH4 production of methanogens over CH4 quantification by using gas chromatography, especially if applied as a high throughput quantification method. Eventually, the method can be universally applied for quantification of CH4 production from psychrophilic, thermophilic and hyperthermophilic hydrogenotrophic methanogens.
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