ArticleMetabolomics : Official journal of the Metabolomic Society
LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels.
Article in Metabolomics : Official journal of the Metabolomic Society. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
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- Preanalytical (Mis)Handling of Plasma Investigated byACS omega · 2024Article
- Discovery of Hypoxanthine and Inosine as Robust Biomarkers for Predicting the Preanalytical Quality of Human Plasma and Serum for Metabolomics.Analytical chemistry · 2024Article
- An Overview of Pre-Analytical Factors Impacting Metabolomics Analyses of Blood Samples.Metabolites · 2024Review
- Anomalous Dynamics of Labile Metabolites in Cold Human Blood Detected UsingAnalytical chemistry · 2023Article
- Assessment of Plasma Tylosin Concentrations: A Comparative Study of Immunoassay, Microbiological Assay, and Liquid Chromatography/Mass Spectrometry.Antibiotics (Basel, Switzerland) · 2023Article
- Instrumental Drift in Untargeted Metabolomics: Optimizing Data Quality with Intrastudy QC Samples.Metabolites · 2023Review
- Effect of serum sample storage temperature on metabolomic and proteomic biomarkers.Scientific reports · 2022Article
- Metabolite Ratios as Quality Indicators for Pre-Analytical Variation in Serum and EDTA Plasma.Metabolites · 2021Article
- From bedside to bench-practical considerations to avoid pre-analytical pitfalls and assess sample quality for high-resolution metabolomics and lipidomics analyses of body fluids.Analytical and bioanalytical chemistry · 2021Review
- Effects of processing conditions on stability of immune analytes in human blood.Scientific reports · 2020Article
- Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics.Metabolites · 2020Review
- Approaches to minimising the epidemiological impact of sources of systematic and random variation that may affect biochemistry assay data in UK Biobank.Wellcome open research · 2020Article
- Pre-Analytical Factors that Affect Metabolite Stability in Human Urine, Plasma, and Serum: A Review.Metabolites · 2019Review
- MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines.Journal of lipid research · 2018Article
- Reproducibility of non-fasting plasma metabolomics measurements across processing delays.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Biobanks in the Era of Digital Medicine.Clinical pharmacology and therapeutics · 2018Article
- Elastic net regularized regression for time-series analysis of plasma metabolome stability under sub-optimal freezing condition.Scientific reports · 2018Article
- Prediction and modeling of pre-analytical sampling errors as a strategy to improve plasma NMR metabolomics data.Bioinformatics (Oxford, England) · 2017Article
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Authors and funding
8 authors.
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Abstract
introductionMetabolome analysis is complicated by the continuous dynamic changes of metabolites in vivo and ex vivo. One of the main challenges in metabolomics is the robustness and reproducibility of results, partially driven by pre-analytical variations.
objectivesThe objective of this study was to analyse the impact of pre-centrifugation time and temperature, and to determine a quality control marker in plasma samples.
methodsPlasma metabolites were measured by gas chromatography-mass spectrometry (GC-MS) and analysed with the MetaboliteDetector software. The metabolites, which were the most labile to pre-analytical variations, were further measured by enzymatic assays. A score was calculated for their use as quality control markers.
resultsThe pre-centrifugation temperature was shown to be critical in the stability of plasma samples and had a significant impact on metabolite concentration profiles. In contrast, pre-centrifugation delay had only a minor impact. Based on the results of this study, whole blood should be kept on wet ice and centrifuged within maximum 3 h as a prerequisite for preparing EDTA plasma samples fit for the purpose of metabolome analysis.
conclusionsWe have established a novel blood sample quality control marker, the LacaScore, based on the ascorbic acid to lactic acid ratio in plasma, which can be used as an indicator of the blood pre-centrifugation conditions, and hence the suitability of the sample for metabolome analyses. This method can be applied in research institutes and biobanks, enabling assessment of the quality of their plasma sample collections.
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