ArticleScientific reports2016
Membrane Topology and Structural Insights into the Peptide Pheromone Receptor ComD, A Quorum-Sensing Histidine Protein Kinase of Streptococcus mutans.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Defining Membrane Protein Topology Using pho-lac Reporter Fusions.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Exploiting Conserved Quorum Sensing Signals in Streptococcus mutans and Streptococcus pneumoniaeMicroorganisms · 2022Article
- Development and utilization of peptide-based quorum sensing modulators in Gram-positive bacteria.Organic & biomolecular chemistry · 2020Review
- Expanding the Vocabulary of Peptide Signals inFrontiers in cellular and infection microbiology · 2019Review
- ClpP is required for proteolytic regulation of type II toxin-antitoxin systems and persister cell formation inAccess microbiology · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Quorum sensing activation by signal pheromone (CSP) in Streptococcus mutans depends on the membrane-associated receptor ComD, which senses the signal and triggers the signaling cascade for bacteriocin production and other cell density-dependent activities. However, the mechanism of the signal recognition via the ComD receptor in this species is nearly unexplored. Here, we show that the membrane domain of the ComD protein forms six transmembrane segments with three extracellular loops, loopA, loopB and loopC. By structural and functional analyses of these extracellular loops, we demonstrate that both loopC and loopB are required for CSP recognition, while loopA plays little role in CSP detection. A deletion or substitution mutation of four residues NVIP in loopC abolishes CSP recognition for quorum sensing activities. We conclude that both loopC and loopB are required for forming the receptor and residues NVIP of loopC are essential for CSP recognition and quorum sensing activation in S. mutans.
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