ArticleScientific reports2016
LAMP Detection Assays for Boxwood Blight Pathogens: A Comparative Genomics Approach.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Rapid detection of cultivated truffle fungi (Applied and environmental microbiology · 2026Article
- Genomics in plant pathogen identification and control.Frontiers in plant science · 2025Review
- A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.Frontiers in plant science · 2025Review
- LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives.Life (Basel, Switzerland) · 2024Review
- Calonectria in the age of genes and genomes: Towards understanding an important but relatively unknown group of pathogens.Molecular plant pathology · 2022Review
- Metagenomic sequencing for detection and identification of the boxwood blight pathogen Calonectria pseudonaviculata.Scientific reports · 2022Article
- Comparative analysis of extracellular proteomes reveals putative effectors of the boxwood blight pathogens, Calonectria henricotiae and C. pseudonaviculata.Bioscience reports · 2021Article
- Analysis of the Taxonomy and Pathogenic Factors ofFrontiers in microbiology · 2021Article
- Mating genes inPersoonia · 2020Article
- Reconsideration of species boundaries and proposed DNA barcodes forStudies in mycology · 2020Article
- Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.Frontiers in cellular and infection microbiology · 2020Review
- A Loop-Mediated Isothermal Amplification Assay for Rapid Detection ofInternational journal of molecular sciences · 2019Article
- Boxwood blight: an ongoing threat to ornamental and native boxwood.Applied microbiology and biotechnology · 2018Review
- Article
- A plant pathology perspective of fungal genome sequencing.IMA fungus · 2017Article
- Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and accurate molecular diagnostic tools are critical to efforts to minimize the impact and spread of emergent pathogens. The identification of diagnostic markers for novel pathogens presents several challenges, especially in the absence of information about population diversity and where genetic resources are limited. The objective of this study was to use comparative genomics datasets to find unique target regions suitable for the diagnosis of two fungal species causing a newly emergent blight disease of boxwood. Candidate marker regions for loop-mediated isothermal amplification (LAMP) assays were identified from draft genomes of Calonectria henricotiae and C. pseudonaviculata, as well as three related species not associated with this disease. To increase the probability of identifying unique targets, we used three approaches to mine genome datasets, based on (i) unique regions, (ii) polymorphisms, and (iii) presence/absence of regions across datasets. From a pool of candidate markers, we demonstrate LAMP assay specificity by testing related fungal species, common boxwood pathogens, and environmental samples containing 445 diverse fungal taxa. This comparative-genomics-based approach to the development of LAMP diagnostic assays is the first of its kind for fungi and could be easily applied to diagnostic marker development for other newly emergent plant pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.