ArticleJournal of biomechanics2016
High seeding density of human chondrocytes in agarose produces tissue-engineered cartilage approaching native mechanical and biochemical properties.
Article in Journal of biomechanics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Eight-Year Clinical, Radiological, and Survival Outcomes of Knee Chondral Lesions Treated With High-Density Autologous Chondrocyte Implantation.Cartilage · 2026Article
- A sulfonated cartilage interpenetrating polymer network reinforces and protects the extracellular matrix of degraded cartilage.Acta biomaterialia · 2026Article
- Molecular signaling pathways in osteoarthritis and biomaterials for cartilage regeneration: a review.Bioengineered · 2025Review
- Evaluation of Engineered Cartilage Composition and Function Using Raman Spectroscopy.Advanced healthcare materials · 2025Article
- Elucidating the Chemistry Behind Thiol-Clickable GelAGE Hydrogels for 3D Culture Applications.Gels (Basel, Switzerland) · 2025Article
- Cartilage structure-inspired nanofiber-hydrogel composite with robust proliferation and stable chondral lineage-specific differentiation function to orchestrate cartilage regeneration for artificial tracheal construction.Bioactive materials · 2025Article
- Physiologic Doses of Transforming Growth Factor-β Improve the Composition of Engineered Articular Cartilage.Tissue engineering. Part A · 2025Article
- Cell mediated reactions create TGF-β delivery limitations in engineered cartilage.Acta biomaterialia · 2024Article
- Hydrogel-Enhanced Autologous Chondrocyte Implantation for Cartilage Regeneration-An Update on Preclinical Studies.Bioengineering (Basel, Switzerland) · 2024Review
- Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement.Biomaterials and biosystems · 2024Article
- Scaffold Using Chitosan, Agarose, Cellulose, Dextran and Protein for Tissue Engineering-A Review.Polymers · 2023Review
- Microcarriers in application for cartilage tissue engineering: Recent progress and challenges.Bioactive materials · 2022Review
- Current understanding of osteoarthritis pathogenesis and relevant new approaches.Bone research · 2022Review
- Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources.Journal of functional biomaterials · 2022Review
- Article
- [Research progress of different cell seeding densities and cell ratios in cartilage tissue engineering].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2022Review
- Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering-A Review of the Literature.Gels (Basel, Switzerland) · 2021Review
- Recent Advancements in 3D Printing of Polysaccharide Hydrogels in Cartilage Tissue Engineering.Materials (Basel, Switzerland) · 2021Review
- 3D Bioprinting of Human Tissues: Biofabrication, Bioinks, and Bioreactors.International journal of molecular sciences · 2021Review
- Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Animal cells have served as highly controllable model systems for furthering cartilage tissue engineering practices in pursuit of treating osteoarthritis. Although successful strategies for animal cells must ultimately be adapted to human cells to be clinically relevant, human chondrocytes are rarely employed in such studies. In this study, we evaluated the applicability of culture techniques established for juvenile bovine and adult canine chondrocytes to human chondrocytes obtained from fresh or expired osteochondral allografts. Human chondrocytes were expanded and encapsulated in 2% agarose scaffolds measuring ∅3-4mm×2.3mm, with cell seeding densities ranging from 15 to 90×10(6)cells/mL. Subsets of constructs were subjected to transient or sustained TGF-β treatment, or provided channels to enhance nutrient transport. Human cartilaginous constructs physically resembled native human cartilage, and reached compressive Young's moduli of up to ~250kPa (corresponding to the low end of ranges reported for native knee cartilage), dynamic moduli of ~950kPa (0.01Hz), and contained 5.7% wet weight (%/ww) of glycosaminoglycans (≥ native levels) and 1.5%/ww collagen. We found that the initial seeding density had pronounced effects on tissue outcomes, with high cell seeding densities significantly increasing nearly all measured properties. Transient TGF-β treatment was ineffective for adult human cells, and tissue construct properties plateaued or declined beyond 28 days of culture. Finally, nutrient channels improved construct mechanical properties, presumably due to enhanced rates of mass transport. These results demonstrate that our previously established culture system can be successfully translated to human chondrocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.