ArticleRNA (New York, N.Y.)2016
Stable association of RNAi machinery is conserved between the cytoplasm and nucleus of human cells.
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 62 citations in OpenAlex.
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- Neuron type-specific mRNA translation programs provide a gateway for memory consolidation.bioRxiv : the preprint server for biology · 2025Article
- Loss of Lamin A leads to the nuclear translocation of AGO2 and compromised RNA interference.Nucleic acids research · 2024Article
- Nuclear miRNAs: Gene Regulation Activities.International journal of molecular sciences · 2024Review
- Nuclear localization of Argonaute 2 is affected by cell density and may relieve repression by microRNAs.Nucleic acids research · 2024Article
- Noncanonical functions of microRNAs in the nucleus.Acta biochimica et biophysica Sinica · 2024Review
- Characterization of Argonaute-containing protein complexes in Leishmania-infected human macrophages.PloS one · 2024Article
- Age and 17β-Estradiol (ENon-coding RNA · 2023Article
- Nuclear Localization of Argonaute is affected by Cell Density and May Relieve Repression by microRNAs.bioRxiv : the preprint server for biology · 2023Article
- Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing.Life science alliance · 2023Article
- RNAi in cell nuclei: potential for a new layer of biological regulation and a new strategy for therapeutic discovery.RNA (New York, N.Y.) · 2023Article
- The Integrator complex regulates microRNA abundance through RISC loading.Science advances · 2023Article
- The Multiplicity of Argonaute Complexes in Mammalian Cells.The Journal of pharmacology and experimental therapeutics · 2023Review
- Small Interfering RNAs Targeting a Chromatin-Associated RNA Induce Its Transcriptional Silencing in Human Cells.Molecular and cellular biology · 2022Article
- miRNA-486-5p: signaling targets and role in non-malignant disease.Cellular and molecular life sciences : CMLS · 2022Review
- Exosomal and Non-Exosomal MicroRNAs: New Kids on the Block for Cancer Therapy.International journal of molecular sciences · 2022Review
- Review
- Extracellular vesicle-based interorgan transport of mitochondria from energetically stressed adipocytes.Cell metabolism · 2021Article
- Impact of scaffolding protein TNRC6 paralogs on gene expression and splicing.RNA (New York, N.Y.) · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Argonaute 2 (AGO2), the catalytic engine of RNAi, is typically associated with inhibition of translation in the cytoplasm. AGO2 has also been implicated in nuclear processes including transcription and splicing. There has been little insight into AGO2's nuclear interactions or how they might differ relative to cytoplasm. Here we investigate the interactions of cytoplasmic and nuclear AGO2 using semi-quantitative mass spectrometry. Mass spectrometry often reveals long lists of candidate proteins, complicating efforts to rigorously discriminate true interacting partners from artifacts. We prioritized candidates using orthogonal analytical strategies that compare replicate mass spectra of proteins associated with Flag-tagged and endogenous AGO2. Interactions with TRNC6A, TRNC6B, TNRC6C, and AGO3 are conserved between nuclei and cytoplasm. TAR binding protein interacted stably with cytoplasmic AGO2 but not nuclear AGO2, consistent with strand loading in the cytoplasm. Our data suggest that interactions between functionally important components of RNAi machinery are conserved between the nucleus and cytoplasm but that accessory proteins differ. Orthogonal analysis of mass spectra is a powerful approach to streamlining identification of protein partners.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.