ArticleAdvanced healthcare materials2016
The Use of Silk as a Scaffold for Mature, Sustainable Unilocular Adipose 3D Tissue Engineered Systems.
Article in Advanced healthcare materials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 78 citations in OpenAlex.
- Advancing preclinical research with reconstructed in vitro skin models mimicking non-healing wounds.International journal of pharmaceutics: X · 2026Review
- New Frontiers in modeling the lipedema microenvironmentFrontiers in cell and developmental biology · 2026Review
- Bioprinting functional fat - adipocytes as the next frontier in tissue engineering, regenerative medicine, and cultivated meat.Frontiers in bioengineering and biotechnology · 2026Review
- Development of vascularized adipose organoids from PBMSCs and their application in evaluating Celastrol's effects.Adipocyte · 2025Article
- Unilocular adipocyte and lipid tracer for immunofluorescent images.Scientific reports · 2025Article
- 2D and 3D In Vitro Co-culture for Cancer and Bone Cell Interaction Studies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development and analysis of scaffold-free adipose spheroids.Adipocyte · 2024Article
- Environmental Obesogens and Their Perturbations in Lipid Metabolism.Environment & health (Washington, D.C.) · 2024Review
- Functionalising silk hydrogels with hetero- and homotypic nanoparticles.RSC advances · 2024Article
- Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases.Theranostics · 2024Review
- Optimization and validation of a fat-on-a-chip model for non-invasive therapeutic drug discovery.Frontiers in bioengineering and biotechnology · 2024Article
- Clinical Applicability of Visible Light-Mediated Cross-linking for Structural Soft Tissue Reconstruction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Development of a More Environmentally Friendly Silk Fibroin Scaffold for Soft Tissue Applications.Journal of functional biomaterials · 2023Article
- Review
- Autologous Human Immunocompetent White Adipose Tissue-on-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Development and characterization of three cell culture systems to investigate the relationship between primary bone marrow adipocytes and myeloma cells.Frontiers in oncology · 2022Article
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- In Vitro Models Mimicking Immune Response in the Skin.Yonsei medical journal · 2021Review
- Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix.Biomimetics (Basel, Switzerland) · 2021Article
- 3D Adipose Tissue Culture Links the Organotypic Microenvironment to Improved Adipogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
There is a critical need for monitoring physiologically relevant, sustainable, human adipose tissues in vitro to gain new insights into metabolic diseases. To support long-term culture, a 3D silk scaffold assisted culture system is developed that maintains mature unilocular adipocytes ex vivo in coculture with preadipocytes, endothelial cells, and smooth muscle cells obtained from small volumes of liquefied adipose samples. Without the silk scaffold, adipose tissue explants cannot be sustained in long-term culture (3 months) due to their fragility. Adjustments to media components are used to tune lipid metabolism and proliferation, in addition to responsiveness to an inflammatory stimulus. Interestingly, patient specific responses to TNFα stimulation are observed, providing a proof-of-concept translational technique for patient specific disease modeling in the future. In summary, this novel 3D scaffold assisted approach is required for establishing physiologically relevant, sustainable, human adipose tissue systems from small volumes of lipoaspirate, making this methodology of great value to studies of metabolism, adipokine-driven diseases, and other diseases where the roles of adipocytes are only now becoming uncovered.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.