ArticlePloS one2016
Elemental Analysis of Bone, Teeth, Horn and Antler in Different Animal Species Using Non-Invasive Handheld X-Ray Fluorescence.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 95 citations in OpenAlex.
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- Nanocrystal Compressive Residual Stresses: A Strategy to Strengthen the Bony Spines of Osteocytic and Anosteocytic Fish.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Fabrication and Characterization of Highly Porous Gyroid Scaffolds Composed of Deproteinized Bone Mineral.Journal of functional biomaterials · 2025Article
- Ways to Measure Metals: From ICP-MS to XRF.Current environmental health reports · 2025Review
- Morphometric, histometric and elemental profile of the metacarpal and metatarsal bones in adult Sanjabi sheep.Veterinary research forum : an international quarterly journal · 2025Article
- On the influence of structural and chemical properties on the elastic modulus of woven bone under healing.Frontiers in bioengineering and biotechnology · 2024Article
- Contribution to Knowledge on Bioapatites: Does Mg Level Reflect the Organic Matter and Water Contents of Enamel?International journal of molecular sciences · 2023Article
- Hydrothermal extraction and physicochemical characterization of biogenic hydroxyapatite nanoparticles from buffalo waste bones for in vivo xenograft in experimental rats.Scientific reports · 2023Article
- Mercury Content in Impacted Wisdom Teeth from Patients of the Legnica-Głogów Copper Area-An In Vitro Pilot Study.Journal of xenobiotics · 2023Article
- Properties and Characterization Techniques of Graphene Modified Asphalt Binders.Nanomaterials (Basel, Switzerland) · 2023Review
- The Localized Ionic Microenvironment in Bone Modelling/Remodelling: A Potential Guide for the Design of Biomaterials for Bone Tissue Engineering.Journal of functional biomaterials · 2023Review
- Biomimetic Mineralization of Tooth Enamel Using Nanocrystalline Hydroxyapatite under Various Dental Surface Pretreatment Conditions.Biomimetics (Basel, Switzerland) · 2022Article
- Post-Mortem Dental Profile as a Powerful Tool in Animal Forensic Investigations-A Review.Animals : an open access journal from MDPI · 2022Review
- Article
- Pollution of Feral Pigeon (Columba livia) Depends on Their Age and Their Health Status.Biological trace element research · 2022Article
- Lead content in soil, plants, rodents, and amphibians in the vicinity of a heating plant's ash waste.Environmental monitoring and assessment · 2021Article
- Properties of Cartilage-Subchondral Bone Junctions: A Narrative Review with Specific Focus on the Growth Plate.Cartilage · 2021Review
- Elemental Distribution in Animal Carpal and Tarsal Bones Using Differences in X-ray Fluorescence Energy.Biological trace element research · 2021Article
- Relationship between heavy metal accumulation and histological alterations in voles from alpine and forest habitats of the West Carpathians.Environmental science and pollution research international · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mineralized tissues accumulate elements that play crucial roles in animal health. Although elemental content of bone, blood and teeth of human and some animal species have been characterized, data for many others are lacking, as well as species comparisons. Here we describe the distribution of elements in horn (Bovidae), antler (Cervidae), teeth and bone (humerus) across a number of species determined by handheld X-ray fluorescence (XRF) to better understand differences and potential biological relevance. A difference in elemental profiles between horns and antlers was observed, possibly due to the outer layer of horns being comprised of keratin, whereas antlers are true bone. Species differences in tissue elemental content may be intrinsic, but also related to feeding habits that contribute to mineral accumulation, particularly for toxic heavy metals. One significant finding was a higher level of iron (Fe) in the humerus bone of elephants compared to other species. This may be an adaptation of the hematopoietic system by distributing Fe throughout the bone rather than the marrow, as elephant humerus lacks a marrow cavity. We also conducted discriminant analysis and found XRF was capable of distinguishing samples from different species, with humerus bone being the best source for species discrimination. For example, we found a 79.2% correct prediction and success rate of 80% for classification between human and non-human humerus bone. These findings show that handheld XRF can serve as an effective tool for the biological study of elemental composition in mineralized tissue samples and may have a forensic application.
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Registered trials
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