Evidence map›Paper›PMID 27196318›Full record

ArticleInvestigative ophthalmology & visual science2016

Vitamin D Induces Global Gene Transcription in Human Corneal Epithelial Cells: Implications for Corneal Inflammation.

Rose Y Reins, Fahmi Mesmar, Cecilia Williams, Alison M McDermott

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Vitamin abnormalities in neuropathic corneal pain.Eye and vision (London, England) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Rose Y ReinsCollege of Optometry University of Houston, Houston, Texas, United States.
Fahmi MesmarDepartment of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, Texas, United States.
Cecilia WilliamsDepartment of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, Texas, United States 3Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Solna, Sweden 4Departme.
Alison M McDermottCollege of Optometry University of Houston, Houston, Texas, United States.
University of Houston · USKarolinska Institutet · SE

Funding

Research Programming ModuleP30EY007551 · NEI · UNIVERSITY OF HOUSTON · PI VALLABH E DAS · 1988 to 2026
$16.4M
Role of Antimicrobial Peptides at the Ocular SurfaceR01EY013175 · NEI · UNIVERSITY OF HOUSTON · PI MCDERMOTT, ALISON M · 2001 to 2016
$4.3M
NEI NIH HHS P30 EY007551NEI NIH HHS R01 EY013175
6 · The paper itself

Abstract

purposeOur previous studies show that human corneal epithelial cells (HCEC) have a functional vitamin D receptor (VDR) and respond to vitamin D by dampening TLR-induced inflammation. Here, we further examined the timing of the cytokine response to combined vitamin D-TLR treatment and used genome-wide microarray analysis to examine the effect of vitamin D on corneal gene expression.

methodsTelomerase-immortalized HCEC (hTCEpi) were stimulated with polyinosinic-polycytidylic acid (poly[I:C]) and 1,25-dihydroxyvitamin D3 (1,25D3) for 2 to 24 hours and interleukin (IL)-8 expression was examined by quantitative (q)PCR and ELISA. Telomerase-immortalized HCEC and SV40-HCEC were treated with 1,25D3 and used in genome-wide microarray analysis. Expression of target genes was validated using qPCR in both cell lines and primary HCEC. For confirmation of IκBα protein, hTCEpi were treated with 1,25D3 for 24 hours and cell lysates used in an ELISA.

resultsTreatment with 1,25D3 increased poly(I:C)-induced IL-8 mRNA and protein expression after 2 to 6 hours. However, when cells were pretreated with 1,25D3 for 24 hours, 1,25D3 decreased cytokine expression. For microarray analysis, 308 genes were differentially expressed by 1,25D3 treatment in hTCEpi, and 69 genes in SV40s. Quantitative (q)PCR confirmed the vitamin D-mediated upregulation of target genes, including nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, α (IκBα). In addition to increased transcript levels, IκBα protein was increased by 28% following 24 hours of vitamin D treatment.

conclusionsMicroarray analysis demonstrates that vitamin D regulates numerous genes in HCEC and influences TLR signaling through upregulation of IκBα. These findings are important in dissecting the role of vitamin D at the ocular surface and highlight the need for further research into the functions of vitamin D and its influence on corneal gene expression.

Indexed as

Transcription, GeneticCells, CulturedEnzyme-Linked Immunosorbent AssayEpithelium, CornealGene Expression RegulationHumansInterleukin-8KeratitisReal-Time Polymerase Chain ReactionReceptors, CalcitriolRNA, MessengerSignal TransductionTissue Array AnalysisVitamin DVitaminsInterleukin-8Receptors, CalcitriolRNA, MessengerVitamin DVitamins

Identifiers

PMID27196318
PMCPMC5995024
OpenAlexW2400673535

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.