ArticlePloS one2016
Nanofiber Alignment Regulates NIH3T3 Cell Orientation and Cytoskeletal Gene Expression on Electrospun PCL+Gelatin Nanofibers.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed.
- Investigating the Cellular Activity and Differential Gene Expression of Human Astrocytes in Interaction with Protein Composite Nanofibers.Neurochemical research · 2026Article
- Influence of silica nanoparticle incorporation on the wettability, oil affinity, and dye removal properties of blow-spun polymeric membranes.Journal of materials science · 2026Article
- The functional regulation of protein composite nanofibers on human astrocyte for neural regeneration.Research square · 2025Article
- Development of a bioactive, piezoelectric PVDF-TrFE scaffold with evaluation of tissue reaction for potential in nerve repair.Biomaterials science · 2025Article
- Article
- Enhanced Cell Proliferation, Migration, and Fibroblast Differentiation with Electrospun PCL-Zinc Scaffolds Coated with Fibroblast-Derived ECM.ACS omega · 2025Article
- Potential of Trilayered Gelatin/Polycaprolactone Nanofibers for Periodontal Regeneration: An In Vitro Study.International journal of molecular sciences · 2025Article
- Electrospun nanofiber scaffolds loaded with copper oxide for repairing traumatic brain injury through restoring copper homeostasis and regulating pyroptosis pathway.Burns & trauma · 2025Article
- Evaluating the efficacy of uniformly designed square mesh resin 3D printed scaffolds in directing the orientation of electrospun PCL nanofibers.Scientific reports · 2024Article
- Characterization of Blow-Spun Polyurethane Scaffolds-Influence of Fiber Alignment and Fiber Diameter on Pericyte Growth.ACS biomaterials science & engineering · 2024Article
- Self-organizing behaviors of cardiovascular cells on synthetic nanofiber scaffolds.APL bioengineering · 2023Article
- Nanofibers in Ocular Drug Targeting and Tissue Engineering: Their Importance, Advantages, Advances, and Future Perspectives.Pharmaceutics · 2023Review
- Investigating the effect of poly (ɛ-caprolactone) nanofibers scaffolds with random, unidirectionally, and radially aligned morphologies on the Fibroblast cell's attachment and growth behavior.Turkish journal of chemistry · 2023Article
- Topographic Orientation of Scaffolds for Tissue Regeneration: Recent Advances in Biomaterial Design and Applications.Biomimetics (Basel, Switzerland) · 2022Review
- Mimicking the Natural Basement Membrane for Advanced Tissue Engineering.Biomacromolecules · 2022Review
- Review
- Micropatterning of acoustic droplet vaporization in acoustically-responsive scaffolds using extrusion-based bioprinting.Bioprinting (Amsterdam, Netherlands) · 2022Article
- Photothermal nanofibres enable safe engineering of therapeutic cells.Nature nanotechnology · 2021Article
- Electrospun Scaffold Micro-Architecture Induces an Activated Transcriptional Phenotype within Tendon Fibroblasts.Frontiers in bioengineering and biotechnology · 2021Article
- Three Dimensional Quercetin-Functionalized Patterned Scaffold: Development, Characterization, andACS omega · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
To examine the influence of substrate topology on the behavior of fibroblasts, tissue engineering scaffolds were electrospun from polycaprolactone (PCL) and a blend of PCL and gelatin (PCL+Gel) to produce matrices with both random and aligned nanofibrous orientations. The addition of gelatin to the scaffold was shown to increase the hydrophilicity of the PCL matrix and to increase the proliferation of NIH3T3 cells compared to scaffolds of PCL alone. The orientation of nanofibers within the matrix did not have an effect on the proliferation of adherent cells, but cells on aligned substrates were shown to elongate and align parallel to the direction of substrate fiber alignment. A microarray of cyotoskeleton regulators was probed to examine differences in gene expression between cells grown on an aligned and randomly oriented substrates. It was found that transcriptional expression of eight genes was statistically different between the two conditions, with all of them being upregulated in the aligned condition. The proteins encoded by these genes are linked to production and polymerization of actin microfilaments, as well as focal adhesion assembly. Taken together, the data indicates NIH3T3 fibroblasts on aligned substrates align themselves parallel with their substrate and increase production of actin and focal adhesion related genes.
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Registered trials
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