Evidence map›Paper›PMID 27195810›Full record

ArticlePloS one2016

Manipulation of Cell Cycle and Chromatin Configuration by Means of Cell-Penetrating Geminin.

Yoshinori Ohno, Kyoko Suzuki-Takedachi, Shin'ichiro Yasunaga, Toshiaki Kurogi, Mimoko Santo, Yoshikazu Masuhiro, Shigemasa Hanazawa, Motoaki Ohtsubo, Kazuhito Naka, Yoshihiro Takihara

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Yoshinori OhnoDepartment of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
Kyoko Suzuki-TakedachiDepartment of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
Shin'ichiro YasunagaDepartment of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
Toshiaki KurogiDepartment of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
Mimoko SantoDepartment of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
Yoshikazu MasuhiroDepartment of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, Kameino, Fujisawa-city, Kanagawa, Japan.
Shigemasa HanazawaDepartment of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, Kameino, Fujisawa-city, Kanagawa, Japan.
Motoaki OhtsuboDepartment of Food and Fermentation Science, Faculty of Food Science and Nutrition, Beppu University, Kita-ishigaki 82, Beppu-city, Oita, Japan.
Kazuhito NakaDepartment of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
Yoshihiro TakiharaDepartment of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
Hiroshima University · JPNihon University · JPBeppu University · JPFukuoka University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Geminin regulates chromatin remodeling and DNA replication licensing which play an important role in regulating cellular proliferation and differentiation. Transcription of the Geminin gene is regulated via an E2F-responsive region, while the protein is being closely regulated by the ubiquitin-proteasome system. Our objective was to directly transduce Geminin protein into cells. Recombinant cell-penetrating Geminin (CP-Geminin) was generated by fusing Geminin with a membrane translocating motif from FGF4 and was efficiently incorporated into NIH 3T3 cells and mouse embryonic fibroblasts. The withdrawal study indicated that incorporated CP-Geminin was quickly reduced after removal from medium. We confirmed CP-Geminin was imported into the nucleus after incorporation and also that the incorporated CP-Geminin directly interacted with Cdt1 or Brahma/Brg1 as the same manner as Geminin. We further demonstrated that incorporated CP-Geminin suppressed S-phase progression of the cell cycle and reduced nuclease accessibility in the chromatin, probably through suppression of chromatin remodeling, indicating that CP-Geminin constitutes a novel tool for controlling chromatin configuration and the cell cycle. Since Geminin has been shown to be involved in regulation of stem cells and cancer cells, CP-Geminin is expected to be useful for elucidating the role of Geminin in stem cells and cancer cells, and for manipulating their activity.

Indexed as

Cell CycleChromatin Assembly and DisassemblyAmino Acid MotifsAnimalsCell Cycle ProteinsChromatinDNA-Binding ProteinsDNA HelicasesDNA ReplicationFibroblast Growth Factor 4FibroblastsGemininGene Expression RegulationHumansK562 CellsMiceCell Cycle ProteinsChromatinDNA-Binding ProteinsDNA HelicasesFgf4 protein, mouseFibroblast Growth Factor 4GemininGmnn protein, mouseNuclear ProteinsProteasome Endopeptidase ComplexRecombinant ProteinsRis2 protein, mouseSmarca4 protein, mouseTranscription FactorsUbiquitin

Identifiers

PMID27195810
PMCPMC4873132
OpenAlexW2401008844

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.