ArticlePloS one2016
A Novel Reporter Rat Strain That Conditionally Expresses the Bright Red Fluorescent Protein tdTomato.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
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- Selection of an Efficient AAV Vector for Robust CNS Transgene Expression.Molecular therapy. Methods & clinical development · 2019Article
- Optogenetic analysis of respiratory neuronal networks in the ventral medulla of neonatal rats producing channelrhodopsin in Phox2b-positive cells.Pflugers Archiv : European journal of physiology · 2019Article
- A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression.Scientific reports · 2019Article
- Ipsilateral-Dominant Control of Limb Movements in Rodent Posterior Parietal Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Genetic Modulation at the Neural Microelectrode Interface: Methods and Applications.Micromachines · 2018Article
- Genetic approaches to access cell types in mammalian nervous systems.Current opinion in neurobiology · 2018Review
- A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering Applications.Tissue engineering. Part C, Methods · 2018Article
- Targeted expression of step-function opsins in transgenic rats for optogenetic studies.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato) in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including their projection fibers and spines without any histological enhancement. In addition, a transgenic rat line (FLAME) that ubiquitously expresses tdTomato was successfully established by injecting intracytoplasmic Cre mRNA into fertilized ova. Our rat reporter system will facilitate connectome studies as well as the visualization of the fine structures of genetically identified cells for long periods both in vivo and ex vivo. Furthermore, FLAME is an ideal model for organ transplantation research owing to improved traceability of cells/tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.