ArticlePloS one2016
Characterization of a DNA Adenine Methyltransferase Gene of Borrelia hermsii and Its Dispensability for Murine Infection and Persistence.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Comparative analysis of Borrelia's Defence mechanisms and their impact on genetic manipulation of low-passage isolates ofCurrent research in microbial sciences · 2026Article
- Optimising Transformation Efficiency inInternational journal of molecular sciences · 2024Article
- Development and validation of systems for genetic manipulation of the Old World tick-borne relapsing fever spirochete, Borrelia duttonii.PLoS neglected tropical diseases · 2024Article
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- PERK-mediated antioxidant response is key for pathogen persistence in ticks.bioRxiv : the preprint server for biology · 2023Article
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- The First Immunocompetent Mouse Model of Strictly Human Pathogen, Borrelia recurrentis.Infection and immunity · 2021Article
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Assessing the Contribution of an HtrA Family Serine Protease DuringFrontiers in cellular and infection microbiology · 2019Article
- DNA Methylation by Restriction Modification Systems Affects the Global Transcriptome Profile in Borrelia burgdorferi.Journal of bacteriology · 2018Article
- Cis-acting DNA elements flanking the variable major protein expression site of Borrelia hermsii are required for murine persistence.MicrobiologyOpen · 2018Article
- Colony formation in solid medium by the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae.Ticks and tick-borne diseases · 2018Article
- Genetic Manipulation of Borrelia Spp.Current topics in microbiology and immunologyReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
DNA methyltransferases have been implicated in the regulation of virulence genes in a number of pathogens. Relapsing fever Borrelia species harbor a conserved, putative DNA methyltransferase gene on their chromosome, while no such ortholog can be found in the annotated genome of the Lyme disease agent, Borrelia burgdorferi. In the relapsing fever species Borrelia hermsii, the locus bh0463A encodes this putative DNA adenine methyltransferase (dam). To verify the function of the BH0463A protein product as a Dam, the gene was cloned into a Dam-deficient strain of Escherichia coli. Restriction fragment analysis subsequently demonstrated that complementation of this E. coli mutant with bh0463A restored adenine methylation, verifying bh0463A as a Dam. The requirement of bh0463A for B. hermsii viability, infectivity, and persistence was then investigated by genetically disrupting the gene. The dam- mutant was capable of infecting immunocompetent mice, and the mean level of spirochetemia in immunocompetent mice was not significantly different from wild type B. hermsii. Collectively, the data indicate that dam is dispensable for B. hermsii viability, infectivity, and persistence.
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