ArticleGenetics2016
Novel Intronic RNA Structures Contribute to Maintenance of Phenotype in Saccharomyces cerevisiae.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Autoregulation of three yeast ribosomal protein genes by splicing inhibition.G3 (Bethesda, Md.) · 2026Article
- Comprehensive analysis of Saccharomyces cerevisiae intron structures in vivo.Nature structural & molecular biology · 2025Article
- Autoregulation of RPL7B by inhibition of a structural splicing enhancer.Nucleic acids research · 2025Article
- Analysis of Fungal Genomes Reveals Commonalities of Intron Gain or Loss and Functions in Intron-Poor Species.Molecular biology and evolution · 2021Article
- Discovery of 17 conserved structural RNAs in fungi.Nucleic acids research · 2021Article
- Differential activation mechanisms of two isoforms of Gcr1 transcription factor generated from spliced and un-spliced transcripts in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Functional and transcriptional profiling of non-coding RNAs in yeast reveal context-dependent phenotypes and in trans effects on the protein regulatory network.PLoS genetics · 2021Article
- Autoregulation of yeast ribosomal proteins discovered by efficient search for feedback regulation.Communications biology · 2020Article
- Plasticity of Mitochondrial DNA Inheritance and its Impact on Nuclear Gene Transcription in Yeast Hybrids.Microorganisms · 2020Article
- Excised linear introns regulate growth in yeast.Nature · 2019Article
- DIP1 modulates stem cell homeostasis in Drosophila through regulation of sisR-1.Nature communications · 2017Article
- Rapid functional and evolutionary changes follow gene duplication in yeast.Proceedings. Biological sciences · 2017Article
- A resource for functional profiling of noncoding RNA in the yeastRNA (New York, N.Y.) · 2017Article
- Extremely fast and incredibly close: cotranscriptional splicing in budding yeast.RNA (New York, N.Y.) · 2017Review
- The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA.Molecular and cellular biology · 2016Article
- Workflow for Genome-Wide Determination of Pre-mRNA Splicing Efficiency from Yeast RNA-seq Data.BioMed research international · 2016Article
- Links between mRNA splicing, mRNA quality control, and intellectual disability.RNA & disease (Houston, Tex.) · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae genome has undergone extensive intron loss during its evolutionary history. It has been suggested that the few remaining introns (in only 5% of protein-coding genes) are retained because of their impact on function under stress conditions. Here, we explore the possibility that novel noncoding RNA structures (ncRNAs) are embedded within intronic sequences and are contributing to phenotype and intron retention in yeast. We employed de novo RNA structure prediction tools to screen intronic sequences in S. cerevisiae and 36 other fungi. We identified and validated 19 new intronic RNAs via RNA sequencing (RNA-seq) and RT-PCR. Contrary to the common belief that excised introns are rapidly degraded, we found that, in six cases, the excised introns were maintained intact in the cells. In another two cases we showed that the ncRNAs were further processed from their introns. RNA-seq analysis confirmed that introns in ribosomal protein genes are more highly expressed when they contain predicted RNA structures. We deleted the novel intronic RNA structure within the GLC7 intron and showed that this region, rather than the intron itself, is responsible for the cell's ability to respond to salt stress. We also showed a direct association between the in cis presence of the intronic RNA and GLC7 expression. Overall, these data support the notion that some introns may have been maintained in the genome because they harbor functional RNA structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.