ArticleScientific reports2016
Correlative nonlinear optical microscopy and infrared nanoscopy reveals collagen degradation in altered parchments.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 64 citations in OpenAlex.
- Nanodomain poling unlocking backward nonlinear light generation in thin film lithium niobate.Nanophotonics (Berlin, Germany) · 2025Article
- Minimally Invasive Vacuum-Aided Extraction Technique for the Lipid Analysis of Historic Parchment.Analytical chemistry · 2024Article
- Saturation of fatty acids in phosphatidic acid uniquely alters transthyretin stability changing morphology and toxicity of amyloid fibrils.Chemistry and physics of lipids · 2023Article
- Determination of Secondary Structure of Proteins by Nanoinfrared Spectroscopy.Analytical chemistry · 2023Article
- Characterization of Bacterial Amyloids by Nano-infrared Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Noninvasive quantitative assessment of collagen degradation in parchments by polarization-resolved SHG microscopy.Science advances · 2021Article
- Infrared and Raman chemical imaging and spectroscopy at the nanoscale.Chemical Society reviews · 2020Article
- The Protective Effect ofAnnals of dermatology · 2020Article
- Revealing the Distribution of Metal Carboxylates in Oil Paint from the Micro- to Nanoscale.Angewandte Chemie (International ed. in English) · 2019Article
- An Anterior Cruciate Ligament Failure Mechanism.The American journal of sports medicine · 2019Article
- Animal species identification in parchments by light.Scientific reports · 2019Article
- Article
- Spectroscopic Imaging at the Nanoscale: Technologies and Recent Applications.Analytical chemistry · 2018Review
- Time-Resolved Photoluminescence Microscopy for the Analysis of Semiconductor-Based Paint Layers.Materials (Basel, Switzerland) · 2017Article
- Purple spot damage dynamics investigated by an integrated approach on a 1244 A.D. parchment roll from the Secret Vatican Archive.Scientific reports · 2017Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper presents the correlative imaging of collagen denaturation by nonlinear optical microscopy (NLO) and nanoscale infrared (IR) spectroscopy to obtain morphological and chemical information at different length scales. Such multiscale correlated measurements are applied to the investigation of ancient parchments, which are mainly composed of dermal fibrillar collagen. The main issue is to characterize gelatinization, the ultimate and irreversible alteration corresponding to collagen denaturation to gelatin, which may also occur in biological tissues. Key information about collagen and gelatin signatures is obtained in parchments and assessed by characterizing the denaturation of pure collagen reference samples. A new absorbing band is observed near the amide I band in the IR spectra, correlated to the onset of fluorescence signals in NLO images. Meanwhile, a strong decrease is observed in Second Harmonic signals, which are a structural probe of the fibrillar organization of the collagen at the micrometer scale. NLO microscopy therefore appears as a powerful tool to reveal collagen degradation in a non-invasive way. It should provide a relevant method to assess or monitor the condition of collagen-based materials in museum and archival collections and opens avenues for a broad range of applications regarding this widespread biological material.
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