ArticleScientific reports2016
The cytosolic Fe-S cluster assembly component MET18 is required for the full enzymatic activity of ROS1 in active DNA demethylation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Plants repress ROS1 expression to attenuate heat-induced transposon burst.Nature plants · 2025Article
- Molecular mechanisms and biological functions of active DNA demethylation in plants.Epigenetics & chromatin · 2025Review
- Article
- Base Excision DNA Repair in Plants:International journal of molecular sciences · 2023Review
- Beyond NPK: Mineral Nutrient-Mediated Modulation in Orchestrating Flowering Time.Plants (Basel, Switzerland) · 2023Review
- NICOTIANAMINE SYNTHASE activity affects nucleolar iron accumulation and impacts rDNA silencing and RNA methylation in Arabidopsis.Journal of experimental botany · 2023Article
- The cytosolic iron-sulphur cluster assembly mechanism in grapevine is one target of a virulent Crinkler effector from Plasmopara viticola.Molecular plant pathology · 2022Article
- Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis.Plant physiology · 2022Article
- Transcriptome analysis of a near-isogenic line and its recurrent parent reveals the role of Pup1 QTL in phosphorus deficiency tolerance of rice at tillering stage.Plant molecular biology · 2022Article
- Article
- PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.Life science alliance · 2022Article
- GSNOR Contributes to Demethylation and Expression of Transposable Elements and Stress-Responsive Genes.Antioxidants (Basel, Switzerland) · 2021Article
- Interaction Between Macro- and Micro-Nutrients in Plants.Frontiers in plant science · 2021Review
- Deciphering Epitranscriptome: Modification of mRNA Bases Provides a New Perspective for Post-transcriptional Regulation of Gene Expression.Frontiers in cell and developmental biology · 2021Review
- Dynamics of DNA Methylation and Its Functions in Plant Growth and Development.Frontiers in plant science · 2021Review
- Interactions between metabolism and chromatin in plant models.Molecular metabolism · 2020Review
- Retro-ElementFrontiers in genetics · 2020Article
- Canonical cytosolic iron-sulfur cluster assembly and non-canonical functions of DRE2 in Arabidopsis.PLoS genetics · 2019Article
- Mechanisms of ROS Regulation of Plant Development and Stress Responses.Frontiers in plant science · 2019Review
- Epigenetics of Modified DNA Bases: 5-Methylcytosine and Beyond.Frontiers in genetics · 2018Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation patterns in plants are dynamically regulated by DNA methylation and active DNA demethylation in response to both environmental changes and development of plant. Beginning with the removal of methylated cytosine by ROS1/DME family of 5-methylcytosine DNA glycosylases, active DNA demethylation in plants occurs through base excision repair. So far, many components involved in active DNA demethylation remain undiscovered. Through a forward genetic screening of Arabidopsis mutants showing DNA hypermethylation at the EPF2 promoter region, we identified the conserved iron-sulfur cluster assembly protein MET18. MET18 dysfunction caused DNA hypermethylation at more than 1000 loci as well as the silencing of reporter genes and some endogenous genes. MET18 can directly interact with ROS1 in vitro and in vivo. ROS1 activity was reduced in the met18 mutant plants and point mutation in the conserved Fe-S cluster binding motif of ROS1 disrupted its biological function. Interestingly, a large number of DNA hypomethylated loci, especially in the CHH context, were identified from the met18 mutants and most of the hypo-DMRs were from TE regions. Our results suggest that MET18 can regulate both active DNA demethylation and DNA methylation pathways in Arabidopsis.
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