ArticleBMC complementary and alternative medicine2016
Screening North American plant extracts in vitro against Trypanosoma brucei for discovery of new antitrypanosomal drug leads.
Article in BMC complementary and alternative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 40 citations in OpenAlex.
- Harnessing nature: a systematic exploration ofPharmaceutical biology · 2026Pooled it
- The anti-Trypanosoma activities of medicinal plants: A systematic review of the literature.Veterinary medicine and science · 2022Pooled it
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- The Ethnopharmacology, Phytochemistry and Bioactivities of thePlants (Basel, Switzerland) · 2023Review
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- Salvia ceratophylla L. from South of Jordan: new insights on chemical composition and biological activities.Natural products and bioprospecting · 2020Article
- Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus.Molecules (Basel, Switzerland) · 2020Article
- Evaluation of the Bioactivities ofEvidence-based complementary and alternative medicine : eCAM · 2019Article
- Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms.Frontiers in microbiology · 2019Article
- Essential Oils of FiveMolecules (Basel, Switzerland) · 2018Article
- Evaluation of Triazole and Isoxazole Derivatives as Potential Anti-infective Agents.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2018Article
- Salvia Species as Sources of Natural Products with Antiprotozoal Activity.International journal of molecular sciences · 2018Review
- Inhibitors of ubiquitin E3 ligase as potential new antimalarial drug leads.BMC pharmacology & toxicology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman African Trypanosomiasis (HAT) is a protozoan parasitic disease caused by Trypanosoma brucei. The disease is endemic in regions of sub-Saharan Africa, covering 36 countries and more than 60 million people at the risk. Only few drugs are available for the treatment of HAT. Current drugs suffer from severe toxicities and require intramuscular or intravenous administrations. The situation is further aggravated due to the emergence of drug resistance. There is an urgent need of new drugs that are effective orally against both stages of HAT. Natural products offer an unmatched source for bioactive molecules with new chemotypes.
methodsThe extracts prepared from 522 plants collected from various parts of the North America were screened in vitro against blood stage trypamastigote forms of T. brucei. Active extracts were further screened at concentrations ranging from 10 to 0.4 μg/mL. Active extracts were also investigated for toxicity in Differentiated THP1 cells at 10 μg/mL concentration. The results were computed for dose-response analysis and determination of IC50/IC90 values.
resultsA significant number (150) of extracts showed >90 % inhibition of growth of trypomastigote blood forms of T. brucei in primary screening at 20 μg/mL concentration. The active extracts were further investigated for dose-response inhibition of T. brucei growth. The antitrypansomal activity of 125 plant extracts was confirmed with IC50 < 10 μg/mL. None of these active extracts showed toxicity against differentiated THP1 cells. Eight plants extracts namely, Alnus rubra, Hoita macrostachya, Sabal minor, Syzygium aqueum, Hamamelis virginiana, Coccoloba pubescens, Rhus integrifolia and Nuphar luteum were identified as highly potent antitrypanosomal extracts with IC50 values <1 μg/mL.
conclusionsLimited phytochemical and pharmacological reports are available for the lead plant extracts with potent antitrypanosomal activity. Follow up evaluation of these plant extracts is likely to yield new antitrypanosomal drug-leads or alternate medicines for treatment of HAT.
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