Evidence map›Paper›PMID 27193862›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Generation and Purification of Tetraploid Cells.

Elizabeth M Shenk, Neil J Ganem

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Critical Evaluation of Methods for the Identification of Aneugens.Environmental and molecular mutagenesis · 2025
    Review
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Elizabeth M ShenkDepartments of Pharmacology & Experimental Therapeutics and Medicine, Division of Hematology and Oncology, Boston University School of Medicine, Boston, MA, 02118, USA.
Neil J GanemDepartments of Pharmacology & Experimental Therapeutics and Medicine, Division of Hematology and Oncology, Boston University School of Medicine, Boston, MA, 02118, USA. nganem@bu.edu.

Funding

TRAINING IN BIOMOLECULAR PHARMACOLOGYT32GM008541 · NIGMS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI FARB, DAVID H · 1997 to 2022
$5.2M
Defining novel pathways that arrest genetically unstable tetraploid cellsR00CA154531 · NCI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI GANEM, NEIL J. · 2013 to 2015
$723k
Defining novel pathways that arrest genetically unstable tetraploid cellsK99CA154531 · NCI · DANA-FARBER CANCER INST · PI GANEM, NEIL J. · 2011 to 2012
$300k
NCI NIH HHS K99 CA154531NCI NIH HHS R00 CA154531NIGMS NIH HHS T32 GM008541
6 · The paper itself

Abstract

Tetraploid cells are genetically unstable and have the capacity to promote the development and/or progression of human malignancies. It is now estimated that ~40 % of all solid tumors have passed through a tetraploid intermediate stage at some point during their development. Understanding the biological characteristics of tetraploid cells that impart oncogenic properties is therefore a highly relevant and fundamentally important aspect of cancer biology. Here, we describe strategies to efficiently generate and purify tetraploid cells for use in cell biological studies.

Indexed as

Cell SeparationMitosisTetraploidyCell CycleCell LineCytokinesisFlow CytometryHumansCentrosomeCytokinesisFUCCIHippoMitotic slippagePolyploid

Identifiers

PMID27193862
PMCPMC5091649

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.