Evidence map›Paper›PMID 27193723›Full record

ArticleDoklady. Biochemistry and biophysics2016

Expression and purification of an engineered human endothelin receptor B in a monomeric form.

A V Mishin, A P Luginina, A P Potapenko, V I Borshchevskiy, V Katritch, E Edelweiss, I S Okhrimenko, V I Gordeliy, V G Cherezov

Abstract read
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In one paragraph

Article in Doklady. Biochemistry and biophysics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 4 countries.

A V MishinMoscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia. mishinalexej@gmal.com.
A P LugininaMoscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
A P PotapenkoMoscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
V I BorshchevskiyMoscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
V KatritchBridge Institute, Department of Chemistry, University of Southern California, Los Angeles, California, 90089, USA.
E EdelweissThe European Molecular Biology Laboratory (EMBL), Grenoble Cedex 9, 38042, France.
I S OkhrimenkoMoscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
V I GordeliyMoscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
V G CherezovMoscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
Moscow Institute of Physics and Technology · RUForschungszentrum Jülich · DEUniversity of Southern California · USCentre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.

Indexed as

AnimalsBaculoviridaeBiphenyl CompoundsBlotting, WesternDipeptidesEndothelin Receptor AntagonistsEndothelinsGenetic EngineeringHumansMembrane ProteinsPeptide FragmentsProtein DenaturationProtein StabilityReceptor, Endothelin BRecombinant Fusion ProteinsSf9 CellsBiphenyl CompoundsDipeptidesEDNRB protein, humanEndothelin Receptor AntagonistsEndothelinsIFITM5 protein, humanIRL 2500Membrane ProteinsPeptide FragmentsReceptor, Endothelin BRecombinant Fusion Proteinssovateltide

Identifiers

PMID27193723
OpenAlexW2402864654

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.