Evidence map›Paper›PMID 27193397›Full record

ArticleProteomics2016

Peptide-based systems analysis of inflammation induced myeloid-derived suppressor cells reveals diverse signaling pathways.

Waeowalee Choksawangkarn, Lauren M Graham, Meghan Burke, Sang Bok Lee, Suzanne Ostrand-Rosenberg, Catherine Fenselau, Nathan J Edwards

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 18 citations in OpenAlex.

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  16. Myeloid-Derived Suppressor Cells.Cancer immunology research · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 3 countries.

Waeowalee ChoksawangkarnDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
Lauren M GrahamDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
Meghan BurkeDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
Sang Bok LeeDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
Suzanne Ostrand-RosenbergDepartment of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD, USA.
Catherine FenselauDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
Nathan J EdwardsDepartment of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, USA.
University of Maryland, College Park · USBurapha University · THGeorgetown University Medical Center · USKorea Advanced Institute of Science and Technology · KRUniversity of Maryland, Baltimore County · US

Funding

Ubiquitinated Proteins in Exosomes of Immune Suppressive Myeloid CellsR01GM021248 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI FENSELAU, CATHERINE C, FUSHMAN, DAVID · 1985 to 2017
$4.6M
APPLICATIONS OF MASS SPECTROMETRY IN PHARMACOLOGYR37GM021248 · NIGMS · UNIVERSITY OF MARYLAND COLLEGE PK CAMPUS · PI FENSELAU, CATHERINE C. · 1992 to 2001
$438k
LTQ Orbitrap tandem mass spectrometerS10RR023383 · NCRR · UNIV OF MARYLAND, COLLEGE PARK · PI FENSELAU, CATHERINE C. · 2007 to 2007
$394k
NCRR NIH HHS S10 RR023383NIGMS NIH HHS R01 GM021248NIGMS NIH HHS R37 GM021248
6 · The paper itself

Abstract

A better understanding of molecular signaling between myeloid-derived suppressor cells (MDSC), tumor cells, T-cells, and inflammatory mediators is expected to contribute to more effective cancer immunotherapies. We focus on plasma membrane associated proteins, which are critical in signaling and intercellular communication, and investigate changes in their abundance in MDSC of tumor-bearing mice subject to heightened versus basal inflammatory conditions. Using spectral counting, we observed statistically significant differential abundances for 35 proteins associated with the plasma membrane, most notably the pro-inflammatory proteins S100A8 and S100A9 which induce MDSC and promote their migration. We also tested whether the peptides associated with canonical pathways showed a statistically significant increase or decrease subject to heightened versus basal inflammatory conditions. Collectively, these studies used bottom-up proteomic analysis to identify plasma membrane associated pro-inflammatory molecules and pathways that drive MDSC accumulation, migration, and suppressive potency.

Indexed as

AnimalsCalgranulin ACalgranulin BCell MovementCells, CulturedChromatography, High Pressure LiquidInflammationMembrane ProteinsMice, Inbred BALB CMyeloid-Derived Suppressor CellsNeoplasmsProteomicsTandem Mass SpectrometryCalgranulin ACalgranulin BMembrane ProteinsS100a8 protein, mouseS100A9 protein, mouseCell migrationDifferential protein expressionImmune suppressionInflammationMass spectrometry - LC-MS/MSMyeloid-derived suppressor cellsSystems biology

Identifiers

PMID27193397
PMCPMC6174082
OpenAlexW2399412458

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.