ArticleMolecular biology reports2016
Molecular cloning and characterization of the MsHSP17.7 gene from Medicago sativa L.
Article in Molecular biology reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Expression profile of cystatin gene family in alfalfa (Medicago sativa L.) related to biotic and abiotic stress response.BMC genomics · 2025Article
- The complete mitochondrial genome and phylogenetic analysis ofFrontiers in plant science · 2025Article
- Alfalfa Responses to Intensive Soil Compaction: Effects on Plant and Root Growth, Phytohormones and Internal Gene Expression.Plants (Basel, Switzerland) · 2024Article
- A small heat shock protein (SlHSP17.3) in tomato plays a positive role in salt stress.Frontiers in plant science · 2024Article
- Identification of QTL and candidate genes associated with biomass yield and Feed Quality in response to water deficit in alfalfa (Frontiers in plant science · 2022Article
- Dynamic changes in the transcriptome landscape ofFrontiers in plant science · 2022Article
- The Chloroplastic Small Heat Shock Protein GeneInternational journal of genomics · 2021Article
- Effects of Heat stress and molecular mitigation approaches in orphan legume, Chickpea.Molecular biology reports · 2020Review
- Overexpression of Medicago sativa LEA4-4 can improve the salt, drought, and oxidation resistance of transgenic Arabidopsis.PloS one · 2020Article
- TheFrontiers in plant science · 2020Article
- TheInternational journal of molecular sciences · 2019Article
- CaHSP16.4, a small heat shock protein gene in pepper, is involved in heat and drought tolerance.Protoplasma · 2019Article
- Improving the Yield and Nutritional Quality of Forage Crops.Frontiers in plant science · 2018Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock proteins (HSPs) are ubiquitous protective proteins that play crucial roles in plant development and adaptation to stress, and the aim of this study is to characterize the HSP gene in alfalfa. Here we isolated a small heat shock protein gene (MsHSP17.7) from alfalfa by homology-based cloning. MsHSP17.7 contains a 477-bp open reading frame and encodes a protein of 17.70-kDa. The amino acid sequence shares high identity with MtHSP (93.98 %), PsHSP17.1 (83.13 %), GmHSP17.9 (74.10 %) and SlHSP17.6 (79.25 %). Phylogenetic analysis revealed that MsHSP17.7 belongs to the group of cytosolic class II small heat shock proteins (sHSP), and likely localizes to the cytoplasm. Quantitative RT-PCR indicated that MsHSP17.7 was induced by heat shock, high salinity, peroxide and drought stress. Prokaryotic expression indicated that the salt and peroxide tolerance of Escherichia coli was remarkably enhanced. Transgenic Arabidopsis plants overexpressing MsHSP17.7 exhibited increased root length of transgenic Arabidopsis lines under salt stress compared to the wild-type line. The malondialdehyde (MDA) levels in the transgenic lines were significantly lower than in wild-type, although proline levels were similar between transgenic and wild-type lines. MsHSP17.7 was induced by heat shock, high salinity, oxidative stress and drought stress. Overexpression analysis suggests that MsHSP17.7 might play a key role in response to high salinity stress.
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