ArticleScientific reports2016
Proteomic profiling of cellulase-aid-extracted membrane proteins for functional identification of cellulose synthase complexes and their potential associated- components in cotton fibers.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- From fibers to flowering to metabolites: unlocking hemp (Cannabis sativa) potential with the guidance of novel discoveries and tools.Journal of experimental botany · 2025Review
- Comparative Transcriptomic Analysis ofPlants (Basel, Switzerland) · 2024Article
- CAGEs are Golgi-localized GT31 enzymes involved in cellulose biosynthesis in Arabidopsis.The Plant journal : for cell and molecular biology · 2022Article
- Biochemical and cytological interactions between callose synthase and microtubules in the tobacco pollen tube.Plant cell reports · 2022Article
- Genomic Architecture and Evolution of theFrontiers in plant science · 2022Article
- In silico and expression analyses of fasciclin-like arabinogalactan proteins reveal functional conservation during embryo and seed development.Plant reproduction · 2019Article
- Fascinating Fasciclins: A Surprisingly Widespread Family of Proteins that Mediate Interactions between the Cell Exterior and the Cell Surface.International journal of molecular sciences · 2018Review
- Evolution, gene expression profiling and 3D modeling of CSLD proteins in cotton.BMC plant biology · 2017Article
- Two-Dimensional Gel Electrophoresis-Based Proteomic Analysis Reveals N-terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo.Scientific reports · 2016Article
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cotton fibers are an excellent model for understanding of cellulose biosynthesis in higher plants. In this study, we determined a high cellulose biosynthesis activity in vitro by optimizing biochemical reaction conditions in cotton fibers. By adding a commercial cellulase enzyme into fibers extraction process, we extracted markedly higher levels of GhCESA1 and GhCESA8 proteins and observed an increase in β-1,4-glucan and β-1,3-glucan products in vitro. LC-MS/MS analysis of anti-GhCESA8-immunoprecipitated proteins showed that 19 proteins could be found in three independent experiments including four CESAs (GhCESA1,2,7,8), five well-known non-CESA proteins, one callose synthase (CALS) and nine novel proteins. Notably, upon the cellulase treatment, four CESAs, one CALS and four novel proteins were measured at relatively higher levels by calculating total peptide counts and distinct peptide numbers, indicating that the cellulase-aid-extracted proteins most likely contribute to the increase in β-glucan products in vitro. These results suggest that the cellulase treatment may aid to release active cellulose synthases complexes from growing glucan chains and make them more amenable to extraction. To our knowledge, it is the first time report about the functional identification of the potential proteins that were associated with plant cellulose and callose synthases complexes by using the cellulase-aided protein extraction.
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