ArticleNano letters2016
Ratiometric Tension Probes for Mapping Receptor Forces and Clustering at Intermembrane Junctions.
Article in Nano letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- SynNotch Receptors for Visualizing Immunoreceptor Force Transmission and Downstream Signaling In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- SynNotch receptors for visualizing immunoreceptor force transmission and downstream signaling in vivo.bioRxiv : the preprint server for biology · 2026Article
- Mechanotransduction through T cell receptors: consensus, controversies and future outlooks.Experimental & molecular medicine · 2026Review
- DNA Origami Tension Sensors (DOTS) for Single-Molecule Force Measurements at Fluid Intermembrane Junctions.Nano letters · 2025Article
- CD4+T-cells create a stable mechanical environment for force-sensitive TCR:pMHC interactions.Nature communications · 2025Article
- Biophysical and Structural Features of αβT-Cell Receptor Mechanosensing: A Paradigmatic Shift in Understanding T-Cell Activation.Immunological reviews · 2025Review
- Mechanotransduction governs CD40 function and underlies X-linked hyper-IgM syndrome.Science advances · 2024Article
- Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors.Nature nanotechnology · 2024Article
- Article
- Mechanical forces amplify TCR mechanotransduction in T cell activation and function.Applied physics reviews · 2024Review
- Measurement of Forces Acting on Single T-Cell Receptors.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Hydrogel-based molecular tension fluorescence microscopy for investigating receptor-mediated rigidity sensing.Nature methods · 2023Article
- DNA Nanotechnology for Investigating Mechanical Signaling in the Immune System.Angewandte Chemie (International ed. in English) · 2023Review
- Catch bond models may explain how force amplifies TCR signaling and antigen discrimination.Nature communications · 2023Article
- Immune-mediated alopecias and their mechanobiological aspects.Cells & development · 2022Review
- Understanding immune signaling using advanced imaging techniques.Biochemical Society transactions · 2022Review
- Recent developments in DNA-based mechanical nanodevices.Chemical communications (Cambridge, England) · 2022Review
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Mechanosurveillance: Tiptoeing T Cells.Frontiers in immunology · 2022Review
- Programmable Mechanically Active Hydrogel-Based Materials.Advanced materials (Deerfield Beach, Fla.) · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Short-range communication between cells is required for the survival of multicellular organisms. One mechanism of chemical signaling between adjacent cells employs surface displayed ligands and receptors that only bind when two cells make physical contact. Ligand-receptor complexes that form at the cell-cell junction and physically bridge two cells likely experience mechanical forces. A fundamental challenge in this area pertains to mapping the mechanical forces experienced by ligand-receptor complexes within such a fluid intermembrane junction. Herein, we describe the development of ratiometric tension probes for direct imaging of receptor tension, clustering, and lateral transport within a model cell-cell junction. These probes employ two fluorescent reporters that quantify both the ligand density and the ligand tension and thus generate a tension signal independent of clustering. As a proof-of-concept, we applied the ratiometric tension probes to map the forces experienced by the T-cell receptor (TCR) during activation and showed the first direct evidence that the TCR-ligand complex experiences sustained pN forces within a fluid membrane junction. We envision that the ratiometric tension probes will be broadly useful for investigating mechanotransduction in juxtacrine signaling pathways.
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