ArticleVirulence2016
The Cek1‑mediated MAP kinase pathway regulates exposure of α‑1,2 and β‑1,2‑mannosides in the cell wall of Candida albicans modulating immune recognition.
Article in Virulence, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
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- Rho2 regulates granulocyte-triggered stress adaptation and cell wall remodeling in Aspergillus fumigatus.Microbiological research · 2025Article
- Hydroxychloroquine an Antimalarial Drug, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Multitargeting.Journal of microbiology (Seoul, Korea) · 2024Article
- The defective gut colonization ofVirulence · 2023Article
- Reverse thiophosphorylase activity of a glycoside phosphorylase in the synthesis of an unnatural Manβ1,4GlcNAc library.Chemical science · 2023Article
- Antibodies as Models and Tools to DecipherJournal of fungi (Basel, Switzerland) · 2023Review
- Potential Anti-International journal of molecular sciences · 2023Article
- When Is It Appropriate to Take Off the Mask? Signaling Pathways That Regulate ß(1,3)-Glucan Exposure inFrontiers in fungal biology · 2022Article
- Activation of Cph1 causes ß(1,3)-glucan unmasking in Candida albicans and attenuates virulence in mice in a neutrophil-dependent manner.PLoS pathogens · 2021Article
- Revisiting the Vital Drivers and Mechanisms of β-Glucan Masking in Human Fungal Pathogen,Pathogens (Basel, Switzerland) · 2021Review
- The Glyoxylate Cycle Is Involved in White-Opaque Switching inJournal of fungi (Basel, Switzerland) · 2021Article
- Hog1 Controls Lipids Homeostasis Upon Osmotic Stress inJournal of fungi (Basel, Switzerland) · 2020Article
- Characterization of aJournal of fungi (Basel, Switzerland) · 2020Article
- Cooperative Role of MAPK Pathways in the Interaction ofMicroorganisms · 2019Article
- Lrg1 Regulates β (1,3)-Glucan Masking in Candida albicans through the Cek1 MAP Kinase Pathway.mBio · 2019Article
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
- Impact of Fungal MAPK Pathway Targets on the Cell Wall.Journal of fungi (Basel, Switzerland) · 2018Article
- Article
- The Hsp90 Chaperone Network Modulates Candida Virulence Traits.Trends in microbiology · 2017Review
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cek1 MAP kinase (MAPK) mediates vegetative growth and cell wall biogenesis in the fungal pathogen Candida albicans. Alterations in the fungal cell wall caused by a defective Cek1‑mediated signaling pathway leads to increased β‑1,3‑glucan exposure influencing dectin‑1 fungal recognition by immune cells. We show here that cek1 cells also display an increased exposure of α‑1,2 and β‑1,2‑mannosides (α‑M and β‑M), a phenotype shared by strains defective in the activating MAPKK Hst7, suggesting a general defect in cell wall assembly. cek1 cells display walls with loosely bound material as revealed by transmission electron microscopy and are sensitive to tunicamycin, an inhibitor of N‑glycosylation. Transcriptomal analysis of tunicamycin treated cells revealed a differential pattern between cek1 and wild type cells which involved mainly cell wall and stress related genes. Mapping α‑M and β‑M epitopes in the mannoproteins of different cell wall fractions (CWMP) revealed an important shift in the molecular weight of the mannan derived from mutants defective in this MAPK pathway. We have also assessed the role of galectin‑3, a member of a β‑galactoside‑binding protein family shown to bind to and kill C. albicans through β‑M recognition, in the infection caused by cek1 mutants. Increased binding of cek1 to murine macrophages was shown to be partially blocked by lactose. Galectin-3(-/-) mice showed increased resistance to fungal infection, although galectin-3 did not account for the reduced virulence of cek1 mutants in a mouse model of systemic infection. All these data support a role for the Cek1‑mediated pathway in fungal cell wall maintenance, virulence and antifungal discovery.
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