ArticlePLoS pathogens2016
Direct and Indirect Targeting of PP2A by Conserved Bacterial Type-III Effector Proteins.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
- Comparative genomic insights into the genus Pantoea: genetic determinants of ecological lifestyle diversity and plant growth-promoting potential.World journal of microbiology & biotechnology · 2026Article
- Alternative splicing: an underexplored layer in immune receptor regulation, systemic resistance and priming.Frontiers in plant science · 2026Article
- Structural Insights Into the Role of RxLR Effectors in the Arms Race Between Oomycetes and Plants.Molecular plant pathology · 2025Review
- The conserved AvrE family of bacterial effectors: functions and targets during pathogenesis.Trends in microbiology · 2025Review
- Extracellular niche establishment by plant pathogens.Nature reviews. Microbiology · 2024Review
- Small holes, big impact: Stomata in plant-pathogen-climate epic trifecta.Molecular plant · 2024Review
- Effector modularity promotes functional diversification and evolutionary processes.Science China. Life sciences · 2023Article
- Protein phosphatase 2A: a high-value target of virulence factors.Trends in parasitology · 2023Article
- Distinct Clades of Protein Phosphatase 2A Regulatory B'/B56 Subunits Engage in Different Physiological Processes.International journal of molecular sciences · 2023Review
- Bacterial pathogens deliver water/solute-permeable channels as a virulence strategy.bioRxiv : the preprint server for biology · 2023Article
- Article
- "B" Regulatory Subunits of PP2A: Their Roles in Plant Development and Stress Reactions.International journal of molecular sciences · 2023Review
- Large-Scale Transposon Mutagenesis Reveals Type III Secretion Effector HopR1 Is a Major Virulence Factor inPlants (Basel, Switzerland) · 2022Article
- Chloroplasts play a central role in facilitating MAMP-triggered immunity, pathogen suppression of immunity and crosstalk with abiotic stress.Plant, cell & environment · 2022Article
- Evolutionarily conserved bacterial effectors hijack abscisic acid signaling to induce an aqueous environment in the apoplast.Cell host & microbe · 2022Article
- Pathogens pulling the strings: Effectors manipulating salicylic acid and phenylpropanoid biosynthesis in plants.Molecular plant pathology · 2021Review
- Characterization of Virulence Function of Pseudomonas cichorii Avirulence Protein E1 (AvrE1) during Host Plant Infection.The plant pathology journal · 2021Article
- What the Wild Things Do: Mechanisms of Plant Host Manipulation by Bacterial Type III-Secreted Effector Proteins.Microorganisms · 2021Review
- Pinpointing regulatory protein phosphatase 2A subunits involved in beneficial symbiosis between plants and microbes.BMC plant biology · 2021Article
- Comparative Transcriptome Analysis of Wheat Lines in the Field Reveals Multiple Essential Biochemical Pathways Suppressed by Obligate Pathogens.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial AvrE-family Type-III effector proteins (T3Es) contribute significantly to the virulence of plant-pathogenic species of Pseudomonas, Pantoea, Ralstonia, Erwinia, Dickeya and Pectobacterium, with hosts ranging from monocots to dicots. However, the mode of action of AvrE-family T3Es remains enigmatic, due in large part to their toxicity when expressed in plant or yeast cells. To search for targets of WtsE, an AvrE-family T3E from the maize pathogen Pantoea stewartii subsp. stewartii, we employed a yeast-two-hybrid screen with non-lethal fragments of WtsE and a synthetic genetic array with full-length WtsE. Together these screens indicate that WtsE targets maize protein phosphatase 2A (PP2A) heterotrimeric enzyme complexes via direct interaction with B' regulatory subunits. AvrE1, another AvrE-family T3E from Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), associates with specific PP2A B' subunit proteins from its susceptible host Arabidopsis that are homologous to the maize B' subunits shown to interact with WtsE. Additionally, AvrE1 was observed to associate with the WtsE-interacting maize proteins, indicating that PP2A B' subunits are likely conserved targets of AvrE-family T3Es. Notably, the ability of AvrE1 to promote bacterial growth and/or suppress callose deposition was compromised in Arabidopsis plants with mutations of PP2A genes. Also, chemical inhibition of PP2A activity blocked the virulence activity of both WtsE and AvrE1 in planta. The function of HopM1, a Pto DC3000 T3E that is functionally redundant to AvrE1, was also impaired in specific PP2A mutant lines, although no direct interaction with B' subunits was observed. These results indicate that sub-component specific PP2A complexes are targeted by bacterial T3Es, including direct targeting by members of the widely conserved AvrE-family.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.