ArticleEvidence-based complementary and alternative medicine : eCAM2016
Methanolic Extract of Clinacanthus nutans Exerts Antinociceptive Activity via the Opioid/Nitric Oxide-Mediated, but cGMP-Independent, Pathways.
Article in Evidence-based complementary and alternative medicine : eCAM, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- The phytochemistry and pharmacological activities ofFrontiers in pharmacology · 2026Review
- Antinociceptive effect of Arvelexin in male mouse models.Scientific reports · 2025Article
- Phytochemical composition ofBioMedicine · 2024Review
- Antinociceptive activity of doliroside B.Pharmaceutical biology · 2023Article
- From Plant to Chemistry: Sources of Active Opioid Antinociceptive Principles for Medicinal Chemistry and Drug Design.Molecules (Basel, Switzerland) · 2023Review
- Article
- Article
- A Narrative Review on the Phytochemistry, Pharmacology and Therapeutic Potentials ofMolecules (Basel, Switzerland) · 2021Review
- Article
- Comparison of diclofenac with apigenin-glycosides rich Clinacanthus nutans extract for amending inflammation and catabolic protease regulations in osteoporotic-osteoarthritis rat model.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2020Article
- Alterations in Herbage Yield, Antioxidant Activities, Phytochemical Contents, and Bioactive Compounds of Sabah Snake Grass (Molecules (Basel, Switzerland) · 2020Article
- Methanol Extract ofBiomolecules · 2020Article
- Article
- Methanol extract of Muntingia calabura leaves attenuates CClPharmaceutical biology · 2019Article
- Antitumor and antioxidant effects of Clinacanthus nutans Lindau in 4 T1 tumor-bearing mice.BMC complementary and alternative medicine · 2019Article
- Antinociceptive activity of petroleum ether fraction obtained from methanolic extract of Clinacanthus nutans leaves involves the activation of opioid receptors and NO-mediated/cGMP-independent pathway.BMC complementary and alternative medicine · 2019Article
- In Vitro Cytotoxic Activity of Clinacanthus nutans Leaf Extracts Against HeLa CellsAsian Pacific journal of cancer prevention : APJCP · 2019Article
- Antinociceptive Activity of Petroleum Ether Fraction ofBioMed research international · 2019Article
- Vermicompost Supplementation Improves the Stability of Bioactive Anthocyanin and Phenolic Compounds inMolecules (Basel, Switzerland) · 2018Article
- A Comprehensive Review on Phytochemistry and Pharmacological Activities ofEvidence-based complementary and alternative medicine : eCAM · 2018Review
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The objectives of the present study were to determine the mechanisms of antinociceptive effect of methanol extract of Clinacanthus nutans (Acanthaceae) leaves (MECN) using various animal nociceptive models. The antinociceptive activity of orally administered 10% DMSO, 100 mg/kg acetylsalicylic acid (ASA), 5 mg/kg morphine, or MECN (100, 250, and 500 mg/kg) was determined using the acetic acid-induced abdominal constriction (ACT), formalin-induced paw licking (FT), and hot plate tests (HPT). The role of opioid and nitric oxide/cyclic guanosine monophosphate (NO/cGMP) systems was also investigated. The results showed that MECN produced a significant (p < 0.05) antinociceptive response in all nociceptive models with the recorded ED50 value of 279.3 mg/kg for the ACT, while, for the early and late phases of the FT, the value was >500 mg/kg or 227.7 mg/kg, respectively. This antinociceptive activity was fully antagonized by naloxone (a nonselective opioid antagonist) but was partially reversed by l-arginine (l-arg; a nitric oxide [NO] precursor), Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; an NO synthase inhibitor), or their combinations thereof. In contrast, 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ; a soluble guanylyl cyclase inhibitor) enhanced the extract's antinociception. UHPLC analysis revealed the presence of several flavonoid-based compounds with antinociceptive action. In conclusion, MECN exerted the peripherally and centrally mediated antinociceptive activity via the modulation of the opioid/NO-mediated, but cGMP-independent, systems.
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Registered trials
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