ArticleAmerican journal of physiology. Lung cellular and molecular physiology2016
Selective and inducible targeting of CD11b+ mononuclear phagocytes in the murine lung with hCD68-rtTA transgenic systems.
Article in American journal of physiology. Lung cellular and molecular physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Pulmonary Immune Cell Landscape Altered by Exposure to HIV,International journal of molecular sciences · 2026Article
- NUDT21 Regulates Macrophage Cytokine Responses via Alternative Polyadenylation in ARDS.Research square · 2025Article
- TREM2 promotes lung fibrosis via controlling alveolar macrophage survival and pro-fibrotic activity.Nature communications · 2025Article
- Myeloid- and Epithelial-derived Heparin-Binding Epidermal Growth Factor-like Growth Factor Promotes Pulmonary Fibrosis.American journal of respiratory cell and molecular biology · 2022Article
- A Human CD68 Promoter-Driven Inducible Cre-Recombinase Mouse Line Allows Specific Targeting of Tissue Resident Macrophages.Frontiers in immunology · 2022Article
- Characterization of a Murine Model System to Study MicroRNA-147 During Inflammatory Organ Injury.Inflammation · 2021Article
- Altered Macrophage Polarization Induces Experimental Pulmonary Hypertension and Is Observed in Patients With Pulmonary Arterial Hypertension.Arteriosclerosis, thrombosis, and vascular biology · 2021Observational
- SPHK2-Generated S1P in CD11bCell reports · 2020Article
- Single cell RNA sequencing identifies unique inflammatory airspace macrophage subsets.JCI insight · 2019Article
- Deletion of c-FLIP from CD11bAmerican journal of respiratory cell and molecular biology · 2018Article
- The cre-inducer doxycycline lowers cytokine and chemokine transcript levels in the gut of mice.Journal of applied genetics · 2017Article
- A Time- and Compartment-Specific Activation of Lung Macrophages in Hypoxic Pulmonary Hypertension.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Promoter Specificity and Efficacy in Conditional and Inducible Transgenic Targeting of Lung Macrophages.Frontiers in immunology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
During homeostasis two distinct macrophage (Mø) populations inhabit the lungs: tissue Mø (often called interstitial Mø) and resident alveolar Mø (resAMø). During acute lung inflammation, monocytes from the circulation migrate to areas of injury where they mature into a third Mø population: recruited Mø. Resident AMø uniquely express low levels of CD11b and high levels of CD11c. In comparison, recruited Mø and tissue Mø express high levels of CD11b and low levels of CD11c. It is likely that these three Mø subpopulations play distinct roles in injury and disease states; however, tools with which to individually target or track these populations are lacking. Here we demonstrate the utility of an hCD68-rtTA transgenic system for specific, robust, and inducible targeting of CD11b(+) recruited Mø and tissue Mø in the murine lung with negligible activation in resAMø. Using hCD68rtTA-GFP reporter mice, we show both during homeostasis and inflammation that administration of doxycycline induces tet-On reporter expression in recruited Mø and tissue Mø but not in resident AMø. We further demonstrate how hCD68-rtTA can be effectively combined with tet-On Cre to target these same recMø and tissue Mø. Accordingly, the hCD68-rtTA system is a powerful new tool that can be used for lineage tracing, fate mapping, and gene deletion in a variety of murine models, thereby enabling sophisticated investigation of the unique role of these CD11b(+) Mø during lung heath and disease.
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