ArticleThe Journal of biological chemistry2016
Generation of Integration-free Induced Neural Stem Cells from Mouse Fibroblasts.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Gestational Hypoxia Disrupts Medial Ganglionic Eminence Progenitor Dynamics and Interneuron Development in Schizophrenia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Study on the therapeutic potential of induced neural stem cells for Alzheimer's disease in mice.Biological research · 2024Article
- POU3F4 up-regulates Gli1 expression and promotes neuronal differentiation and synaptic development of hippocampal neural stem cells.Stem cell research & therapy · 2024Article
- Research progress of the transcription factor Brn4 (Review).Molecular medicine reports · 2021Article
- Generation of Porcine Induced Neural Stem Cells Using the Sendai Virus.Frontiers in veterinary science · 2021Article
- Take the shortcut - direct conversion of somatic cells into induced neural stem cells and their biomedical applications.FEBS letters · 2019Review
- Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons.International journal of stem cells · 2019Article
- Plasmid-based generation of neural cells from human fibroblasts using non-integrating episomal vectors.Neural regeneration research · 2019Article
- Evolving principles underlying neural lineage conversion and their relevance for biomedical translation.F1000Research · 2019Review
- Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery.Journal of nanobiotechnology · 2017Article
- Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages.In vitro cellular & developmental biology. Animal · 2017Article
- Reprogramming Enhancers in Somatic Cell Nuclear Transfer, iPSC Technology, and Direct Conversion.Stem cell reviews and reports · 2017Review
- Therapeutic Potential of Induced Neural Stem Cells for Parkinson's Disease.International journal of molecular sciences · 2017Article
- Plasmid-Based Generation of Induced Neural Stem Cells from Adult Human Fibroblasts.Frontiers in cellular neuroscience · 2016Article
Corrections and comments
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Authors and funding
17 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The viral vector-mediated overexpression of the defined transcription factors, Brn4/Pou3f4, Sox2, Klf4, and c-Myc (BSKM), could induce the direct conversion of somatic fibroblasts into induced neural stem cells (iNSCs). However, viral vectors may be randomly integrated into the host genome thereby increasing the risk for undesired genotoxicity, mutagenesis, and tumor formation. Here we describe the generation of integration-free iNSCs from mouse fibroblasts by non-viral episomal vectors containing BSKM. The episomal vector-derived iNSCs (e-iNSCs) closely resemble control NSCs, and iNSCs generated by retrovirus (r-iNSCs) in morphology, gene expression profile, epigenetic status, and self-renewal capacity. The e-iNSCs are functionally mature, as they could differentiate into all the neuronal cell types both in vitro and in vivo Our study provides a novel concept for generating functional iNSCs using a non-viral, non-integrating, plasmid-based system that could facilitate their biomedical applicability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.