Evidence map›Paper›PMID 27189541›Full record

ArticleMolecular biology and evolution2016

Evolution of Vertebrate Phototransduction: Cascade Activation.

Trevor D Lamb, Hardip Patel, Aaron Chuah, Riccardo C Natoli, Wayne I L Davies, Nathan S Hart, Shaun P Collin, David M Hunt

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 55 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Trevor D LambEccles Institute of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia trevor.lamb@anu.edu.au.
Hardip PatelGenome Discovery Unit, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia Department of Genome Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
Aaron ChuahGenome Discovery Unit, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
Riccardo C NatoliEccles Institute of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia ANU Medical School, Australian National University, Canberra, ACT, Australia.
Wayne I L DaviesSchool of Animal Biology, University of Western Australia, Perth, WA, Australia Oceans Institute, University of Western Australia, Perth, WA, Australia Lions Eye Institute, University of Western Australia, Perth, WA, Australia.
Nathan S HartSchool of Animal Biology, University of Western Australia, Perth, WA, Australia Oceans Institute, University of Western Australia, Perth, WA, Australia Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Shaun P CollinSchool of Animal Biology, University of Western Australia, Perth, WA, Australia Oceans Institute, University of Western Australia, Perth, WA, Australia Lions Eye Institute, University of Western Australia, Perth, WA, Australia.
David M HuntSchool of Animal Biology, University of Western Australia, Perth, WA, Australia Lions Eye Institute, University of Western Australia, Perth, WA, Australia.
Australian National University · AUUniversity of Western Australia · AULions Eye Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We applied high-throughput sequencing to eye tissue from several species of basal vertebrates (a hagfish, two species of lamprey, and five species of gnathostome fish), and we analyzed the mRNA sequences for the proteins underlying activation of the phototransduction cascade. The molecular phylogenies that we constructed from these sequences are consistent with the 2R WGD model of two rounds of whole genome duplication. Our analysis suggests that agnathans retain an additional representative (that has been lost in gnathostomes) in each of the gene families we studied; the evidence is strong for the G-protein α subunit (GNAT) and the cGMP phosphodiesterase (PDE6), and indicative for the cyclic nucleotide-gated channels (CNGA and CNGB). Two of the species (the hagfish Eptatretus cirrhatus and the lamprey Mordacia mordax) possess only a single class of photoreceptor, simplifying deductions about the composition of cascade protein isoforms utilized in their photoreceptors. For the other lamprey, Geotria australis, analysis of the ratios of transcript levels in downstream and upstream migrant animals permits tentative conclusions to be drawn about the isoforms used in four of the five spectral classes of photoreceptor. Overall, our results suggest that agnathan rod-like photoreceptors utilize the same GNAT1 as gnathostomes, together with a homodimeric PDE6 that may be agnathan-specific, whereas agnathan cone-like photoreceptors utilize a GNAT that may be agnathan-specific, together with the same PDE6C as gnathostomes. These findings help elucidate the evolution of the vertebrate phototransduction cascade from an ancestral chordate phototransduction cascade that existed prior to the vertebrate radiation.

Indexed as

AnimalsBiological EvolutionEvolution, MolecularEyeFishesGenomeGlucosidesGTP-Binding Protein alpha SubunitsHigh-Throughput Nucleotide SequencingLampreysLight Signal TransductionPhenolsPhylogenyRetinal Cone Photoreceptor CellsGlucosidesGTP-Binding Protein alpha SubunitsPhenolsrhodiolosidecyclic nucleotide-gated channel.phosphodiesterasephototransductionrhodopsintransducin

Identifiers

PMID27189541
PMCPMC4948711
OpenAlexW2355837210

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.