ArticleNature communications2016
Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
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- A Novel Fractional Brownian Dynamics Method for Simulating the Dynamics of Confined Bottle-Brush Polymers in Viscoelastic Solution.Polymers · 2024Article
- SMORES: A Simple Microfluidic Operating Room for the Examination and Surgery ofbioRxiv : the preprint server for biology · 2024Article
- Quantitative microbiology with widefield microscopy: navigating optical artefacts for accurate interpretations.Npj imaging · 2024Article
- Structure of phosphorylated-like RssB, the adaptor delivering σThe Journal of biological chemistry · 2023Article
- Synthetic Micrographs of Bacteria (SyMBac) allows accurate segmentation of bacterial cells using deep neural networks.BMC biology · 2022Article
- Microfluidic dose-response platform to track the dynamics of drug response in single mycobacterial cells.Scientific reports · 2022Article
- Quantifying biochemical reaction rates from static population variability within incompletely observed complex networks.PLoS computational biology · 2022Article
- Next generation single-molecule techniques: Imaging, labeling, and manipulation in vitro and in cellulo.Molecular cell · 2022Review
- Dynamics of Proteins and Macromolecular Machines in Escherichia coli.EcoSal Plus · 2021Review
- Cellular heterogeneity in DNA alkylation repair increases population genetic plasticity.Nucleic acids research · 2021Article
- Lab-on-a-chip based mechanical actuators and sensors for single-cell and organoid culture studies.Journal of applied physics · 2021Article
- Tracking bacterial lineages in complex and dynamic environments with applications for growth control and persistence.Nature microbiology · 2021Article
- Mesoscale Simulation of Bacterial Chromosome and Cytoplasmic Nanoparticles in Confinement.Entropy (Basel, Switzerland) · 2021Article
- Challenges of analysing stochastic gene expression in bacteria using single-cell time-lapse experiments.Essays in biochemistry · 2021Article
- Slipstreaming Mother Machine: A Microfluidic Device for Single-Cell Dynamic Imaging of Yeast.Micromachines · 2020Article
- Article
Corrections and comments
- Erratum issuedCorrigendum: Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells.2016 · Bakhsi, Somenath [corrected to Bakshi, Somenath]
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
Many key regulatory proteins in bacteria are present in too low numbers to be detected with conventional methods, which poses a particular challenge for single-cell analyses because such proteins can contribute greatly to phenotypic heterogeneity. Here we develop a microfluidics-based platform that enables single-molecule counting of low-abundance proteins by mechanically slowing-down their diffusion within the cytoplasm of live Escherichia coli (E. coli) cells. Our technique also allows for automated microscopy at high throughput with minimal perturbation to native physiology, as well as viable enrichment/retrieval. We illustrate the method by analysing the control of the master regulator of the E. coli stress response, RpoS, by its adapter protein, SprE (RssB). Quantification of SprE numbers shows that though SprE is necessary for RpoS degradation, it is expressed at levels as low as 3-4 molecules per average cell cycle, and fluctuations in SprE are approximately Poisson distributed during exponential phase with no sign of bursting.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.