ArticleScientific reports2016
Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed.
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- Time-dependent effects of rapid-acting antidepressants in iPSC-derived neurons from treatment-resistant depression and healthy volunteers.Molecular psychiatry · 2026Article
- Suppression of ATM kinase signaling accelerates cellular senescence.Stem cell reports · 2026Article
- Microelectrode Arrays Technology for Brain-on-a-Chip Applications.Biosensors · 2026Review
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- Hierarchical Afferent Connectivity Drives Population-Wide Bursting Dynamics in a Computational Model of Human-Derived Excitatory Neuronal Networks.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines.Cell reports methods · 2026Article
- Article
- Forward programming of human pluripotent stem cells to generate glutamatergic and GABAergic neurons in a tri-culture model with astrocytes.Stem cell research & therapy · 2026Article
- Time-Dependent Effects of Rapid-Acting Antidepressants in iPSC-Derived Neurons from Treatment-Resistant Depression and Healthy Volunteers.Research square · 2026Article
- Context-tuned strategies for marker selection precision in neuronal studies.Frontiers in neuroscience · 2026Review
- Electrical stimulation of stem cell-derived human neural networks for evaluating anti-seizure medications.Epilepsia · 2025Article
- Investigating the ageing-Parkinson's disease nexus: standardisation of in vitro models and techniques by the PD-AGE network.NPJ Parkinson's disease · 2025Review
- Long-term potentiation and long-term depression are both impaired afterBiomedical physics & engineering express · 2025Article
- Induction of retinal progenitors and neurons from dental follicle stem cell under defined conditions.Journal of dental sciences · 2025Article
- How is Excitotoxicity Being Modelled in iPSC-Derived Neurons?Neurotoxicity research · 2024Review
- Strategies for modeling aging and age-related diseases.npj aging · 2024Review
- Exploring Kainic Acid-Induced Alterations in Circular Tripartite Networks with Advanced Analysis Tools.eNeuro · 2024Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
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Abstract
The functional network of human induced pluripotent stem cell (hiPSC)-derived neurons is a potentially powerful in vitro model for evaluating disease mechanisms and drug responses. However, the culture time required for the full functional maturation of individual neurons and networks is uncertain. We investigated the development of spontaneous electrophysiological activity and pharmacological responses for over 1 year in culture using multi-electrode arrays (MEAs). The complete maturation of spontaneous firing, evoked responses, and modulation of activity by glutamatergic and GABAergic receptor antagonists/agonists required 20-30 weeks. At this stage, neural networks also demonstrated epileptiform synchronized burst firing (SBF) in response to pro-convulsants and SBF suppression using clinical anti-epilepsy drugs. Our results reveal the feasibility of long-term MEA measurements from hiPSC-derived neuronal networks in vitro for mechanistic analyses and drug screening. However, developmental changes in electrophysiological and pharmacological properties indicate the necessity for the international standardization of culture and evaluation procedures.
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