ArticleExperimental biology and medicine (Maywood, N.J.)2016
Nanoallergens: A multivalent platform for studying and evaluating potency of allergen epitopes in cellular degranulation.
Article in Experimental biology and medicine (Maywood, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Dendrimeric Antigens for Passive Mast Cell Activation in the Evaluation of Amoxicillin Allergy.Allergy · 2026Article
- Engineering nanoallergens to investigate origins of immunogenicity and cross-reactivity in cephalosporin allergy.The Journal of allergy and clinical immunology · 2025Article
- Determination of immunogenic epitopes in major house dust mite allergen, Der p 2, via nanoallergens.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2022Article
- Review
- Nanoarchitectures for efficient IgE cross-linking on effector cells to study amoxicillin allergy.Allergy · 2021Article
- Article
- Interplay between Affinity and Valency in Effector Cell Degranulation: A Model System with Polcalcin Allergens and Human Patient-Derived IgE Antibodies.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Covalent Heterobivalent Inhibitor Design for Inhibition of IgE-Dependent Penicillin Allergy in a Murine Model.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Nanoallergen platform for detection of platin drug allergies.The Journal of allergy and clinical immunology · 2019Article
- Designer covalent heterobivalent inhibitors prevent IgE-dependent responses to peanut allergen.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- WHO/IUIS Allergen Nomenclature: Providing a common language.Molecular immunology · 2018Review
- Determination of Crucial Immunogenic Epitopes in Major Peanut Allergy Protein, Ara h2, via Novel Nanoallergen Platform.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Degranulation caused by type I hypersensitivity (allergies) is a complex biophysical process, and available experimental models for studying relevant immunoglobulin E binding epitopes on allergen proteins lack the ability to adequately evaluate, rank, and associate these epitopes individually and with each other. In this study, we propose a new allergy model system for studying potential allergen epitopes using nanoallergens, liposomes modified to effectively display IgE binding epitopes/haptens. By utilizing the covalently conjugated lipid tails on two hapten molecules (dinitrophenol and dansyl), hapten molecules were successfully incorporated into liposomes with high precision to form nanoallergens. Nanoallergens, with precisely controlled high-particle valency, can trigger degranulation with much greater sensitivity than commonly used bovine serum albumin conjugates. In rat basophil leukemia cell experiments, nanoallergens with only 2% hapten loading were able to trigger degranulation in vitro at concentrations as low as 10 pM. Additionally, unlike bovine serum albumin-hapten conjugates, nanoallergens allow exact control over particle size and valency. By varying the nanoallergen parameters such as size, valency, monovalent affinity of hapten, and specific IgE ratios, we exposed the importance of these variables on degranulation intensity while demonstrating nanoallergens' potential for evaluating both high- and low-affinity epitopes. The data presented in this article establish nanoallergen platform as a reliable and versatile allergy model to study and evaluate allergen epitopes in mast cell degranulation.
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