ArticleNature communications2016
Flow cytometry combined with viSNE for the analysis of microbial biofilms and detection of microplastics.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 105 citations in OpenAlex.
- Gaps between Laboratory Experiments and Real-World Exposure: Toxicological Assessment of Microplastics Is Based on Inadequate Evidence.Environment & health (Washington, D.C.) · 2026Review
- A particle-resolved framework for quantifying microbial colonization and vector risk on environmental microplastics.Eco-Environment & Health · 2026Article
- Detection and Fate of Microplastics and Nanoplastics and Technologies for Their Removal.Molecules (Basel, Switzerland) · 2026Review
- Orthodontic derived microplastics impact macrophage differentiation and homeostasis.Progress in orthodontics · 2026Article
- Microplastics and nanoplastics detection using flow cytometry: Challenges and methodological advances with fluorescent dye application.MethodsX · 2025Review
- Integrating biophysical modeling, quantum computing, and AI to discover plastic-binding peptides that combat microplastic pollution.PNAS nexus · 2025Article
- Biofilm formation on microplastics and interactions with antibiotics, antibiotic resistance genes and pathogens in aquatic environment.Eco-Environment & Health · 2024Review
- Article
- Ionic Liquid-Assisted Thermal Evaporation of Bimetallic Ag-Au Nanoparticle Films as a Highly Reproducible SERS Substrate for Sensitive Nanoplastic Detection in Complex Environments.Analytical chemistry · 2024Article
- Generation of Simulated "Natural" Nanoplastics from Polypropylene Food Packaging as the Experimental Standard.Molecules (Basel, Switzerland) · 2023Article
- Geometry Regularized Autoencoders.IEEE transactions on pattern analysis and machine intelligence · 2023Article
- Microplastics: A Matter of the Heart (and Vascular System).Biomedicines · 2023Review
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- Screening for polystyrene nanoparticle toxicity on kidneys of adult male albino rats using histopathological, biochemical, and molecular examination results.Cell and tissue research · 2022Article
- A microfluidic chip enables fast analysis of water microplastics by optical spectroscopy.Scientific reports · 2021Article
- Computational Analysis of Microbial Flow Cytometry Data.mSystems · 2021Review
- Alterations in B cell subsets correlate with body composition parameters in female adolescents with anorexia nervosa.Scientific reports · 2021Article
- Immune signatures of prodromal multiple sclerosis in monozygotic twins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Toxicity of Microplastics and Nanoplastics in Mammalian Systems.International journal of environmental research and public health · 2020Review
- Characterization of Aquatic Biofilms with Flow Cytometry.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilms serve essential ecosystem functions and are used in different technical applications. Studies from stream ecology and waste-water treatment have shown that biofilm functionality depends to a great extent on community structure. Here we present a fast and easy-to-use method for individual cell-based analysis of stream biofilms, based on stain-free flow cytometry and visualization of the high-dimensional data by viSNE. The method allows the combined assessment of community structure, decay of phototrophic organisms and presence of abiotic particles. In laboratory experiments, it allows quantification of cellular decay and detection of survival of larger cells after temperature stress, while in the field it enables detection of community structure changes that correlate with known environmental drivers (flow conditions, dissolved organic carbon, calcium) and detection of microplastic contamination. The method can potentially be applied to other biofilm types, for example, for inferring community structure for environmental and industrial research and monitoring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.