ArticleInternational journal of molecular sciences2016
Hairpin RNA Targeting Multiple Viral Genes Confers Strong Resistance to Rice Black-Streaked Dwarf Virus.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- A Review of Vector-Borne Rice Viruses.Viruses · 2022Review
- Foliar Infiltration of Virus-Derived Small Hairpin RNAs Triggers the RNAi Mechanism against the Cucumber Mosaic Virus.International journal of molecular sciences · 2022Article
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Development of marker-free rice with stable and high resistance to rice black-streaked dwarf virus disease through RNA interference.Plant biotechnology journal · 2021Article
- Alterations of Rice (International journal of molecular sciences · 2020Article
- Rice black-streaked dwarf virus: From multiparty interactions among plant-virus-vector to intermittent epidemics.Molecular plant pathology · 2020Article
- Symptoms and yield loss caused by rice stripe mosaic virus.Virology journal · 2019Article
- Screening of a multi-virus resistant RNAi construct in cowpea through transient vacuum infiltration method.Virusdisease · 2019Article
- Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants.Scientific reports · 2018Article
- Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection.Frontiers in microbiology · 2017Review
- Expression Analysis of Hairpin RNA CarryingBioMed research international · 2017Article
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice black-streaked dwarf virus (RBSDV) belongs to the genus Fijivirus in the family of Reoviridae and causes severe yield loss in rice-producing areas in Asia. RNA silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering virus resistance in plants, including rice. In this study, we generated transgenic rice lines harbouring a hairpin RNA (hpRNA) construct targeting four RBSDV genes, S1, S2, S6 and S10, encoding the RNA-dependent RNA polymerase, the putative core protein, the RNA silencing suppressor and the outer capsid protein, respectively. Both field nursery and artificial inoculation assays of three generations of the transgenic lines showed that they had strong resistance to RBSDV infection. The RBSDV resistance in the segregating transgenic populations correlated perfectly with the presence of the hpRNA transgene. Furthermore, the hpRNA transgene was expressed in the highly resistant transgenic lines, giving rise to abundant levels of 21-24 nt small interfering RNA (siRNA). By small RNA deep sequencing, the RBSDV-resistant transgenic lines detected siRNAs from all four viral gene sequences in the hpRNA transgene, indicating that the whole chimeric fusion sequence can be efficiently processed by Dicer into siRNAs. Taken together, our results suggest that long hpRNA targeting multiple viral genes can be used to generate stable and durable virus resistance in rice, as well as other plant species.
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