Evidence map›Paper›PMID 27186539›Full record

ArticleJournal of medical signals and sensors

Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells.

Zahra Arab-Bafrani, Daryoush Shahbazi-Gahrouei, Mahdi Abbasian, Mehrafarin Fesharaki

Abstract read
In one paragraph

Article in Journal of medical signals and sensors. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zahra Arab-BafraniStem cell Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran; Department of Medical Physics-Clinical Biochemistry, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Daryoush Shahbazi-GahroueiDepartment of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Mahdi AbbasianDepartment of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Mehrafarin FesharakiDepartment of Cell Sciences Research Center Medical Science, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (SF) of irradiated cells. The estimation of SF based on the cell-growth curve information is the major advantage of this assay. In this study, the utility of multiple-MTS assay for the SF estimation of irradiated HT-29 colon cancer cells, which were plated before irradiation, was evaluated. The SF of HT-29 colon cancer cells under irradiation with 9 MV photon was estimated using multiple-MTS assay and colony assay. Finally, the correlation between two assays was evaluated. Results showed that there are no significant differences between the SF obtained by two assays at different radiation doses (P > 0.05), and the survival curves have quite similar trends. In conclusion, multiple MTS-assay can be a reliable method to determine the SF of irradiated colon cancer cells that plated before irradiation.

Indexed as

Colon cancer cellscolony assaymultiple-MTS assayplating before irradiation

Identifiers

PMID27186539
PMCPMC4855884

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.