ArticleBrain and behavior2016
In vivo injection of α-bungarotoxin to improve the efficiency of motor endplate labeling.
Article in Brain and behavior, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 16 citations in OpenAlex.
- Whole-Mount Optical Clearing of Rabbit Tenuissimus Muscle for Assessment of Muscle Spindle Morphology.bioRxiv : the preprint server for biology · 2026Article
- Whole-mount optical clearing of rabbit tenuissimus muscle for assessment of muscle spindle morphology.Frontiers in neuroscience · 2026Article
- Abnormalities in the genioglossus muscle and its neuromuscular synapse in leptin-deficient male mice.Skeletal muscle · 2025Article
- Review
- Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.Neural regeneration research · 2022Article
- Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research.Neural regeneration research · 2022Article
- Skeletal muscle sympathetic denervation disrupts the neuromuscular junction postterminal organization: A single-cell quantitative approach.Molecular and cellular neurosciences · 2022Article
- Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle.Neural regeneration research · 2022Article
- Article
- An Approach to Maximize Retrograde Transport Based on the Spatial Distribution of Motor Endplates in Mouse Hindlimb Muscles.Frontiers in cellular neuroscience · 2021Article
- Studies on the Manner of Collateral Regeneration From Nerve Stem to Motor Endplate.Frontiers in physiology · 2021Article
- A Novel Optical Tissue Clearing Protocol for Mouse Skeletal Muscle to Visualize Endplates in Their Tissue Context.Frontiers in cellular neuroscience · 2019Article
- Spatial Distribution of Motor Endplates and its Adaptive Change in Skeletal Muscle.Theranostics · 2019Article
- In vivo injection of α-bungarotoxin to improve the efficiency of motor endplate labeling.Brain and behavior · 2016Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionMotor endplates are composed of a motor neuron terminal and muscle fiber and are distributed in skeletal muscle, causing muscle contraction. However, traditional motor endplate staining methods are limited to the observation of partial skeletal muscle. The procedure was time-consuming due to strict incubation conditions, and usually provided unsatisfactory results. We explored a novel method to label motor endplate rapidly by in vivo injection of fluorescent α-bungarotoxin.
methodsFifty-two mice were randomly divided into two groups, an experiment group (n = 50), and a contrast group (n = 2). In experiment group, α-bungarotoxin was injected via the caudal vein. The injection dosages were designated as 0.1, 0.2, 0.3, 0.4, and 0.5 μg/g. The experimental mice were divided into five subgroups of ten mice per group. The contrast group was only injected with 200 μL normal saline solution. Bilateral gastrocnemius were acquired for microscope analysis and optical clearing to seek specific fluorescent signal.
resultsA dose of 0.3 μg/g of α-bungarotoxin with 1 h conjugation time could display the number and structure of motor endplate in plane view. Compared with the traditional procedure, this method was rapid, convenient, and time-saving. Combined with the optical clearing technique, spatial distribution could also be seen, helping to better understand the stereoscopic view of motor endplate position in skeletal muscle.
conclusionsIn vivo injection of α-bungarotoxin proved effective for studying motor endplate in skeletal muscle.
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