ArticleProceedings of the National Academy of Sciences of the United States of America2016
Mammalian African trypanosome VSG coat enhances tsetse's vector competence.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- MicroRNA Responses Associated with Vector-Borne Diseases in Humans and Arthropod Vectors: A Systematic Review.International journal of molecular sciences · 2026Pooled it
- PGRP-LA regulates peritrophic matrix synthesis and influences trypanosome infection outcomes in tsetse flies.PLoS pathogens · 2026Article
- mir-31 mediated control of bacteriome size in tsetse flies.Current research in insect science · 2025Article
- Spiroplasma endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly Glossina fuscipes.PLoS pathogens · 2025Article
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- A volatile sex attractant of tsetse flies.Science (New York, N.Y.) · 2023Article
- Symbiosis preservation: Putative regulation of fatty acyl-CoA reductase by miR-31a within the symbiont harboring bacteriome through tsetse evolution.Frontiers in microbiology · 2023Article
- The Tsetse Metabolic Gambit: Living on Blood by Relying on Symbionts Demands Synchronization.Frontiers in microbiology · 2022Review
- The developmentally dynamic microRNA transcriptome ofBioinformatics advances · 2022Article
- Role of Virulence Factors of Trypanosomatids in the Insect Vector and Putative Genetic Events Involved in Surface Protein Diversity.Frontiers in cellular and infection microbiology · 2022Review
- Tick gut barriers impacting tick-microbe interactions and pathogen persistence.Molecular microbiology · 2021Review
- Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly's midgut environment.PLoS pathogens · 2021Article
- Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus.Nature microbiology · 2020Article
- Circulatory microRNAs: promising non-invasive prognostic and diagnostic biomarkers for parasitic infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Single-cell RNA sequencing ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Tsetse peritrophic matrix influences for trypanosome transmission.Journal of insect physiology · 2019Review
- Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes.Genome biology · 2019Article
- Colonization of the tsetse fly midgut with commensal Kosakonia cowanii Zambiae inhibits trypanosome infection establishment.PLoS pathogens · 2019Article
- The Tsetse Fly Displays an Attenuated Immune Response to Its Secondary Symbiont,Frontiers in microbiology · 2019Article
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9 authors at 2 institutions in 1 country.
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Abstract
Tsetse flies are biological vectors of African trypanosomes, the protozoan parasites responsible for causing human and animal trypanosomiases across sub-Saharan Africa. Currently, no vaccines are available for disease prevention due to antigenic variation of the Variant Surface Glycoproteins (VSG) that coat parasites while they reside within mammalian hosts. As a result, interference with parasite development in the tsetse vector is being explored to reduce disease transmission. A major bottleneck to infection occurs as parasites attempt to colonize tsetse's midgut. One critical factor influencing this bottleneck is the fly's peritrophic matrix (PM), a semipermeable, chitinous barrier that lines the midgut. The mechanisms that enable trypanosomes to cross this barrier are currently unknown. Here, we determined that as parasites enter the tsetse's gut, VSG molecules released from trypanosomes are internalized by cells of the cardia-the tissue responsible for producing the PM. VSG internalization results in decreased expression of a tsetse microRNA (mir-275) and interferes with the Wnt-signaling pathway and the Iroquois/IRX transcription factor family. This interference reduces the function of the PM barrier and promotes parasite colonization of the gut early in the infection process. Manipulation of the insect midgut homeostasis by the mammalian parasite coat proteins is a novel function and indicates that VSG serves a dual role in trypanosome biology-that of facilitating transmission through its mammalian host and insect vector. We detail critical steps in the course of trypanosome infection establishment that can serve as novel targets to reduce the tsetse's vector competence and disease transmission.
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